This free course was developed to provide a succinct, contextual overview of basic T cell biology and applications for studying T cells in the laboratory.
The T Cell Stimulation and Proliferation eLearning Course comprises two learning modules and a practical application module.
Learning module part 1 covers these aspects of T cell biology in translational biomedical science:
Learning module part 2 covers T cell isolation and in vitro activation, assessment of T cell activation, proliferation, and differentiation by these methods:
Practical Application module gives you the opportunity to design a multiparameter flow cytometric experiment to assess T cell activity, using what you have learned in Parts 1 and 2.
After completion of the T Cell Stimulation and Proliferation eLearning Course, you should be able to:
Below is an example of the content within the T Cell Stimulation and Proliferation Course.
Here we present an example of the content from the T Cell Stimulation and Proliferation eLearning course.
T cells are primarily involved in adaptive rather than innate immune responses. Adaptive immunity is mediated by both CD4 and CD8-positive T cells and by antibody-producing B cells. Adaptive immunity is a relatively slow response triggered by the recognition antigens. Importantly, the adaptive immune response improves with time and results in the generation of immunological memory and long-lasting protection.
The innate immune response provides for a rapid first-line of defense against infection and is promoted by a diverse array of immune cell types shown here. Innate immunity is not dependent upon prior antigen exposure and does not result in immunological memory.
Following antigen exposure (Figure 1), stimulated T cells undergo clonal expansion and differentiate into effector cells that manifest effector function through cell-mediated cytotoxicity or cytokine secretion. In order to proliferate, differentiate, and express effector function, activated T cells undergo a significant shift in phenotype compared to their naïve counterparts.
This shift involves the induction of a myriad of factors including effector cytokines, lineage-restricted transcription factors, and surface markers such as co-stimulatory molecules, immune checkpoint proteins, adhesion molecules, chemokine receptors, and other receptor proteins.
For Research Use Only. Not for use in diagnostic procedures.