FGF-basic is a critical component for maintaining embryonic stem cells in culture in an undifferentiated state. Gibco Recombinant Human FGF-Basic is a bioactive protein intended for use in cell culture applications. Gibco FGF-Basic comes in three forms: 1) FGF-basic (AA 1-155), full-length version, and 2) FGF-basic (AA 10-155), truncated version, and 3) Heat Stable FGF-basic.
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BALB/3T3 cells (mouse fibroblast cell line, ATCC: CCL-163) at constant density were co-cultured with serial dilutions of FGF-basic in serum-free medium at 37°C, 5% CO2 for 18–24 hours. The optical density (OD) of each concentration of the testing samples, which is directly proportional to the cell number, was read at 490/650 nm at the endpoint of incubation. The cell proliferation dose curve was plotted with mean OD vs. FGF-basic concentrations (ng/mL).
Figure 1. ED50 of recombinant human FGF-basic (AA 1-155) Gibco (blue): 0.02 ng/mL; competitor (red): 0.16 ng/mL.
References
- Eiselleova, L et al. (2009) A Complex Role for FGF-2 in Self-Renewal, Survival, and Adhesion of Human Embryonic Stem Cells. Stem Cells 27(8): 1847–1857
- Karumbayaram S et al. (2009) Human embryonic stem cell-derived motor neurons expressing SOD1 mutants exhibit typical signs of motor neuron degeneration linked to ALS. Dis Model Mech 189–195.
- Zhou J et al. (2009) mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells. Proc Natl Acad Sci USA 106:7840–7845.
- Lu S et al. (2008) Biologic properties and enucleation of red blood cells from human embryonic stem cells. Blood 112:4475–4484.
