NOTE: This map applies only to our new and improved pSilencer 3.1-H1 puro vector which began shipping in Jan 2004. Please Click Here to see the map and sequence information for the previous version of this vector.

pSilencer™ 3.1-H1 puro Vector Map

pSilencer™ 3.1-H1 puro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 3.1-H1 puro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'




pSilencer™ 3.1-H1 puro Restriction Sites (Vector Size: 4121 bp)

Enzymes with single Restriction Sites

Site Enzyme
3252AccI
916AflIII
3082AgeI
1809AhdI
1332AlwNI
3031AvrII
495BamHI
3053BmtI
3870BsaBI
3027BseRI
3199BsiWI
3959BsmI
3056BspMI
3598BaaHII
3277BstEII
3714DraIII
4110EcoO109I
396EcoRI
557HindIII
3971HpaI
3958MfeI
3518MscI
184NdeI
3049NheI
916PciI
2727PflMI
3067PstI
3259RsrII
3357SacII
3251SalI
800SapI
3067SbfI
2289ScaI
2984SfiI
2613SspI
3185Tth111I
3859XbaI
2408XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BclI
BglII
BlpI
BmgBI
Bpu10I
BsaAI
BsgI
BspEI
BsrGI
BstBI
BstXI
BstZ17I
Bsu36I
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
KpnI
MluI
NaeI
NgoMIV
NotI
NruI
PacI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SanDI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XcmI
XhoI
Stylesheet for Classic Wide Template adjustments
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