NOTE: This map applies only to our new and improved pSilencer 3.1-H1 puro vector which began shipping in Jan 2004. Please Click Here to see the map and sequence information for the previous version of this vector.

pSilencer™ 3.1-H1 puro Vector Map

pSilencer™ 3.1-H1 puro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 3.1-H1 puro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'





pSilencer™ 3.1-H1 puro Restriction Sites (Vector Size: 4121 bp)

Enzymes with single Restriction Sites

Site Enzyme
3252 AccI
916 AflIII
3082 AgeI
1809 AhdI
1332 AlwNI
3031 AvrII
495 BamHI
3053 BmtI
3870 BsaBI
3027 BseRI
3199 BsiWI
3959 BsmI
3056 BspMI
3598 BaaHII
3277 BstEII
3714 DraIII
4110 EcoO109I
396 EcoRI
557 HindIII
3971 HpaI
3958 MfeI
3518 MscI
184 NdeI
3049 NheI
916 PciI
2727 PflMI
3067 PstI
3259 RsrII
3357 SacII
3251 SalI
800 SapI
3067 SbfI
2289 ScaI
2984 SfiI
2613 SspI
3185 Tth111I
3859 XbaI
2408 XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BclI
BglII
BlpI
BmgBI
Bpu10I
BsaAI
BsgI
BspEI
BsrGI
BstBI
BstXI
BstZ17I
Bsu36I
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
KpnI
MluI
NaeI
NgoMIV
NotI
NruI
PacI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SanDI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XcmI
XhoI