pSilencer™ 4.1-CMV hygro Vector Map

pSilencer™ 4.1-CMV hygro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.


pSilencer 4.1-CMV hygro Annotated Promoter/Cloning Site Region



pSilencer 4.1-CMV hygro Restriction Sites

Enzymes with single Restriction Sites

Site Enzyme
405 BntI
2528 BsaI
1121 BseRI
3447 BsmI
4363 BspMI
3393 BstZ17I
5198 EcoO109I
462 HindIII
235 KasI
1114 KpnI
3580 NaeI
236 NarI
3578 NgoMIV
401 NheI
406 NotI
1574 PciI
4653 PshAI
3506 PsiI
4347 PstI
4269 RsrII
3902 SacII
3384 SalI
1458 SapI
4972 SexAI
237 SfoI
4318 SgfI
4721 SmaI
763 SnaBI
4745 StuI
4719 XmaI
3066 XmnI
Enzyme
BsaBI
BsgI
BsiWI
BsrGI
BssHII
BstBI
BstEII
BstXI
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
HpaI
MfeI
MluI
MscI
NruI
PacI
PflMI
PmeI
PpuMI
PspOMI
PsrI
PsrI'
SacI
SanDI
SbfI
SfiI
SgrAI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI