pSilencer 4.1-CMV neo Vector Map

pSilencer 4.1-CMV neo Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.

pSilencer 4.1-CMV neo Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

Forward: 5'-AGGCGATTAAGTTGGGTA-3'

Reverse: 5'-CGGTAGGCGTGTACGGTG-3'





pSilencer 4.1-CMV neo Restriction Sites

Enzymes with single Restriction Sites

Site Enzyme
1110 Acc65I
1574 AflIII
2467 AhdI
539 AloI
507 AloI`
1990 AlwNI
4431 AvaI
4452 AvrII
515 BanHI
3885 BanII
2972 BcgI
3006 BcgI`
4401 BclI
1104 BglII
405 BmtI
4387 BsaBI
2528 BsaI
1449 BsaXI
1419 BsaXI`
45 BsmBI
3326 BsmI
3845 BssHII
4933 EcoO109I
1118 EcoRI
462 HindIII
1114 KpnI
4164 MscI
401 NheI
406 NotI
3562 NruI
1574 PciI
3385 PsiI
4197 PstI
3727 RsrII
2947 ScaI
4684 SexAI
4433 SmaI
763 SnaBI
4457 StuI
4127 Tth111I
4431 XmaI
3066 XmnI

Non-Cutting Enzymes:

Enzyme
AarI
AccI
AfeI
AflII
AgeI
AleI
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BlpI
BmgBI
Bpu10I
BsgI
BsiWI
BspEI
BsrGI
BstBI
BstEII
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
DraIII
EcoICRI
EcoNI
EcoRV
FseI
FspAI
HincII
HpaI
MfeI
MluI
PacI
PflMI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SacII
SalI
SanDI
SbfI
SfiI
SgfI
SgrAI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI