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pSilencer™ 4.1-CMV puro
pSilencer 4.1-CMV puro Vector Map
This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.
pSilencer 4.1-CMV puro Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
Forward: 5'-AGGCGATTAAGTTGGGTA-3'
Reverse: 5'-CGGTAGGCGTGTACGGTG-3'
pSilencer 4.1-CMV puro Restriction Sites
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 1110 | Acc65I |
| 4245 | AccI |
| 1574 | AflIII |
| 4413 | AgeI |
| 2467 | AhdI |
| 539 | AloI |
| 507 | AloI` |
| 1990 | AlwNI |
| 4464 | AvrII |
| 515 | BamHI |
| 1104 | BglII |
| 763 | BsaAI |
| 3629 | BsaBI |
| 4296 | BsiWI |
| 3542 | BsmI |
| 4439 | BspMI |
| 3897 | BssHII |
| 4217 | BstEII |
| 3788 | DraIII |
| 1118 | EcoRI |
| 462 | HindIII |
| 3528 | HpaI |
| 1114 | KpnI |
| 3537 | MfeI |
| 3981 | MscI |
| 406 | NotI |
| 1574 | PciI |
| 3508 | PsiI |
| 4436 | PstI |
| 4237 | RsrII |
| 4144 | SacII |
| 4244 | SalI |
| 1458 | SapI |
| 4436 | SbfI |
| 2947 | ScaI |
| 4518 | SfiI |
| 4433 | SmaI |
| 763 | SnaBI |
| 3271 | SspI |
| 4313 | Tth111I |
| 3636 | XbaI |
| 3066 | XmnI |
Non-Cutting Enzymes :
| Enzyme |
|---|
| AarI |
| AfeI |
| AflII |
| AleI |
| ApaI |
| AscI |
| AsiSI |
| BaeI |
| BaeI' |
| BbsI |
| BbvCI |
| BclI |
| BlpI |
| BmgBI |
| Bpu10I |
| BsgI |
| BspEI |
| BsrGI |
| BstBI |
| BstXI |
| BstZ17I |
| Bsu36I |
| ClaI |
| EcoICRI |
| EcoNI |
| EcoO109I |
| EcoRV |
| FalI |
| FalI' |
| FseI |
| FspAI |
| MluI |
| NaeI |
| NgoMIV |
| NruI |
| PacI |
| PflMI |
| PmeI |
| PmlI |
| PpuMI |
| PshAI |
| PspOMI |
| PsrI |
| PsrI' |
| SacI |
| SanDI |
| SgrAI |
| SpeI |
| SrfI |
| SwaI |
| TaqII' |
| XcmI |
| XhoI |
Stylesheet for Classic Wide Template adjustments
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