Access an overview of real-time PCR instrument choices and learn how real-time PCR differs from endpoint PCR. How can you use real-time PCR in your research? What is the difference between TaqMan® chemistry and SYBR® Green chemistry?
What should you consider when planning your gene expression assays? What is the impact of multitranscript assays and assay specificity? How do you design custom assays?
What is normalization and why is it important? What are the types of normalizers and what are their functions? How does multigene normalization work?
What is multiplexing, and when and how do you use it?
Discussion topics include setting up your real-time PCR reaction, and preparing master mixes and reagents for plate additions.
Perform dynamic range testing with standard curves, quantify your data using the ΔΔCt method, and gain a better understanding of absolute vs. relative quantification.
Classes are held weekly in our state-of-the-art training centers, online, or in your lab.
For Research Use Only. Not for use in diagnostic procedures.