Western blotting webinars

Watch our learning series includes poster presentations, on-demand webinars (lectures) to receive firsthand knowledge of the latest technologies for protein gel electrophoresis and western blotting.

Featured webinar

Alok Tomar

A novel method for quantitative western blotting that eliminates errors from the use of housekeeping proteins

Presented by: Alok Tomar
Date: September 22, 2020
Time: 11:00 am PDT, 1:00 pm CST

The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent years, journal editors and reviewers have requested adoption of methods that improve the accuracy and reproducibility of quantitative western blot data. Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blot data. Total protein normalization is considered the gold standard for quantitative western blotting. Protein normalization of western blots has relied upon housekeeping proteins, which exhibit signal saturation and varying levels of cellular expression. These issues can produce spurious results, leading to erroneous conclusions. In this webinar, we will talk about a new protein labeling reagent that offers an attractive alternative to achieve total protein normalization. The Invitrogen No-Stain Protein Labeling Reagent is a flexible, accurate, rapid, and reliable method to visualize and normalize proteins on a gel or on a membrane (post-transfer). The No-Stain reagent can covalently bind to all proteins on gels or membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any UV, blue light, or fluorescence imagers. The No-Stain reagent does not require any special gels or reagents and is compatible with existing gel staining and western blotting workflows. We present data and analysis demonstrating the ease of use and improved accuracy and reproducibility of quantitative western blot data with No-Stain Protein Labeling Reagent compared to housekeeping proteins.

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Protein gel electrophoresis

Importance of sample preparation and choosing the right gel for SDS-PAGE

This webinar will discuss the importance of sample preparation in SDS-PAGE electrophoresis and how to choose the right gel chemistry for specific research application.

Choosing the right protein gel for your research application

This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications and how to introduce the right new gel chemistry in your research workflow.

Detecting low abundant proteins in a western blot: Overcoming challenges for improved signal-to-noise of low expressing and precious sample targets

Detection of low-abundance proteins and/or detection of proteins from limited volume samples can be a major challenge when performing western blotting. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal-to-noise of low-abundance proteins for more successful western blot detection.

Western blotting

Light up your western blots – fluorescent western blotting tips, tricks & more

This webinar will highlight the fundamentals of fluorescent western blotting and discuss how to successfully design fluorescent western blotting experiments.

Poster presentation: Alternative blotting methods

This poster presentation will discuss the traditional wet blotting transfer technique as compared to dry and semi-dry transfer techniques.

Complete and innovative western workflow solutions: filling the gap between art and science (presented in Chinese)

This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, highlight our popular products, and introduce tips and tricks for superior western analysis to make western blotting more efficient and effective.

Classical and modernized western blotting

This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, in the framework of the classical approach and the modernized twists that make western blotting more efficient and effective.

Chemiluminescent western blot detection: Bright and bold detection no matter how scarce your target

In this webinar we will discuss the best methods for optimizing this technique as well as how to determine the appropriate reagents for your sensitive target detection.