Detecting low abundant proteins in a western blot: Overcoming challenges for improved signal-to-noise of low expressing and precious sample targets
Presented by: Emily Halbrader, Product Manager, Protein and Cell Analysis
Date: November 5, 2019
Time: 7:00am PDT, 9:00am CST
Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the protein itself is expressed at low levels within the source or is difficult to extract (and thus recovery is low), the sample is limited (leaving a scarce overall volume available to load onto the gel), or because of a combination of multiple conditions. The outcome in such situations is often faint or undetectable band(s) during the imaging step, resulting in inconclusive data analysis. This can lead researchers to repeat the western blot and/or reoptimize the conditions, both of which waste time and resources. In this webinar, we will discuss specific methods from sample preparation through immunodetection that can help overcome challenges and improve signal to noise of low-abundance proteins for more successful western blot detection.
Protein gel electrophoresis
Importance of sample preparation and choosing the right gel for SDS-PAGE
This webinar will discuss the importance of sample preparation in SDS-PAGE electrophoresis and how to choose the right gel chemistry for specific research application.
Choosing the right protein gel for your research application
This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications and how to introduce the right new gel chemistry in your research workflow.
Light up your western blots – fluorescent western blotting tips, tricks & more
This webinar will highlight the fundamentals of fluorescent western blotting and discuss how to successfully design fluorescent western blotting experiments.
Poster presentation: Alternative blotting methods
This poster presentation will discuss the traditional wet blotting transfer technique as compared to dry and semi-dry transfer techniques.
Complete and innovative western workflow solutions: filling the gap between art and science (presented in Chinese)
This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, highlight our popular products, and introduce tips and tricks for superior western analysis to make western blotting more efficient and effective.
Classical and modernized western blotting
This webinar will cover the electrophoresis, transfer, and detection steps of the western blotting workflow, in the framework of the classical approach and the modernized twists that make western blotting more efficient and effective.
Chemiluminescent western blot detection: Bright and bold detection no matter how scarce your target
In this webinar we will discuss the best methods for optimizing this technique as well as how to determine the appropriate reagents for your sensitive target detection.
For Research Use Only. Not for use in diagnostic procedures.