Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Feature | Nuclease Protection Assays | Northern Blotting | RT-PCR | In Situ Hybridization |
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Relative and Absolute Quantitation | Yes | Yes | Yes | Yes |
Detection Limit (Copies of mRNA) | 4,000-5,000 | 10,000 | 1 (theoretical) | Medium |
Level of Optimization Required | Medium | Medium | High | High |
Sample Size | 100 µg max | 30 µg max | Not critical | Tissue sections |
Detect Multiple Transcripts | Yes, in same tube | Yes, generally requires stripping and reprobing | Yes, in same tube | No |
Localizes mRNA Expression within Tissues/Cells | No | No | No | Yes |
Mapping Studies | Yes | No | No | No |
Resolve Comigrating mRNAs | Yes | No | Yes | No |
Sizing mRNAs | No | Yes | No | No |
Detection of Alternatively Spliced Transcripts | No | Yes | No | No |
Distinguish Between Members of Multi-Gene Families | Yes | Only if they are different sizes | Only if they are characterized | No |
Tolerates Partially Degraded RNA | Yes | No | Yes | Yes |
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