CaptureSelect® Affinity Products

CaptureSelect® products possess a combination of unique properties, including selectivity, affinity, and stability, that provide competitive benefits: single-step purification, ease of use, minimized cost of purification, effective impurity removal, higher-quality product, and increased flexibility in the purification process.

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These features make CaptureSelect® products the right tools for small-scale protein purification. The products are specific for a wide variety of target proteins and are therefore well suited to a variety of applications:

  • Small-scale protein purification—ready-to-use affinity resins, including:
  • Antibody Toolbox® products for the separation of immunoglobulin formats from a wide range of environments, such as plasma, milk from transgenic animals, or supernatant from mammalian cell cultures
  • Proteomics Toolbox® products for protein depletion to support biomarker research applications
  • HPLC analytical chromatography columns—rapid, sensitive, and precise quantitation and small-scale sample preparation of proteins from complex mixtures
  • Custom ligand design—the development of antibody ligands against a specific protein or impurity, enabling affinity purification tailored to novel applications

Most biomolecules can be purified with one of these products. Unlike the common bacterial coat protein components proteins A, G, and L, CaptureSelect® affinity ligands are developed with defined specificity and high avidity to the target, enabling the creation of affinity chromatography resins well suited for single-step protein purification (Tables 1 and 2).

  POROS® CaptureSelect® analytical columns Antibody Toolbox® Products Protein purification products Proteomics Toolbox® Products Conjugated ligands

Antibody analytics

  • Quantitation
  • PTM analysis


    

Antibody purification

  • Small scale
 
   

Protein purification

  • Natural/recombinant
  • Affinity tag
  


  
Biomarker discovery     
Instrumentation neededHPLC/FPLCFPLCFPLCFPLC Spin columnsELISA western SPR, BLI


BLI: bio-layer interferometry; PTM: post-translational modifications; SPR: surface plasmon resonance.

 

Table 1. Comparison of CaptureSelect® affinity products.
POROS® protein A, G resins POROS® CaptureSelect® ligands
Target type Prot A Prot G IgG Fc Kappa Lambda IgG-CH1 IgM
IgG1, IgG2, IgG4      
IgG3      
IgG, CH2 domain
deleted
       
IgM       
IgA, IgD, IgE      
Fab, F(ab´)2      
IgG Fc fusion protein     
Free light chains     

 

Table 2. POROS® CaptureSelect® analytical columns.