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CaptureSelect® Affinity Products
CaptureSelect® products possess a combination of unique properties, including selectivity, affinity, and stability, that provide competitive benefits: single-step purification, ease of use, minimized cost of purification, effective impurity removal, higher-quality product, and increased flexibility in the purification process.
These features make CaptureSelect® products the right tools for small-scale protein purification. The products are specific for a wide variety of target proteins and are therefore well suited to a variety of applications:
- Small-scale protein purification—ready-to-use affinity resins, including:
- Antibody Toolbox® products for the separation of immunoglobulin formats from a wide range of environments, such as plasma, milk from transgenic animals, or supernatant from mammalian cell cultures
- Proteomics Toolbox® products for protein depletion to support biomarker research applications
- HPLC analytical chromatography columns—rapid, sensitive, and precise quantitation and small-scale sample preparation of proteins from complex mixtures
- Custom ligand design—the development of antibody ligands against a specific protein or impurity, enabling affinity purification tailored to novel applications
Most biomolecules can be purified with one of these products. Unlike the common bacterial coat protein components proteins A, G, and L, CaptureSelect® affinity ligands are developed with defined specificity and high avidity to the target, enabling the creation of affinity chromatography resins well suited for single-step protein purification (Tables 1 and 2).
| POROS® CaptureSelect® analytical columns | Antibody Toolbox® Products | Protein purification products | Proteomics Toolbox® Products | Conjugated ligands | |
|---|---|---|---|---|---|
Antibody analytics
| • • | • | |||
Antibody purification
| • | ||||
Protein purification
| • | ||||
| Biomarker discovery | • | ||||
| Instrumentation needed | HPLC/FPLC | FPLC | FPLC | FPLC Spin columns | ELISA western SPR, BLI |
BLI: bio-layer interferometry; PTM: post-translational modifications; SPR: surface plasmon resonance.
Table 1. Comparison of CaptureSelect® affinity products.
| POROS® protein A, G resins | POROS® CaptureSelect® ligands | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Target type | Prot A | Prot G | IgG Fc | Kappa | Lambda | IgG-CH1 | IgM | ||||
| IgG1, IgG2, IgG4 | • | • | • | • | • | • | |||||
| IgG3 | • | • | • | • | • | ||||||
| IgG, CH2 domain deleted | • | • | • | • | |||||||
| IgM | • | • | • | ||||||||
| IgA, IgD, IgE | • | • | |||||||||
| Fab, F(ab´)2 | • | • | • | ||||||||
| IgG Fc fusion protein | • | • | • | ||||||||
| Free light chains | • | • | |||||||||
Table 2. POROS® CaptureSelect® analytical columns.
For Research Use Only. Not for use in diagnostic procedures.