Technical Resources

31672 - Chromosome Medium 1A

* note: FBS given in mL / L. Penicillin and Heparin given in units. Streptomycin given in ug / L.

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 174.0 94.5 0.54310346
L-Cystine 240.0 18.0 0.075
L-Glutamine 146.0 292.0 2.0
L-Histidine hydrochloride-H2O 210.0 315.0 1.5
L-Isoleucine 131.0 39.0 0.2977099
L-Leucine 131.0 39.0 0.2977099
L-Lysine hydrochloride 146.0 54.0 0.369863
L-Methionine 149.0 11.0 0.0738255
L-Phenylalanine 165.0 24.0 0.14545454
L-Threonine 119.0 36.0 0.30252102
L-Tryptophan 204.0 7.5 0.036764707
L-Tyrosine 181.0 27.0 0.14917128
L-Tyrosine 181.0 27.0 0.14917128
L-Valine 117.0 35.0 0.2991453
Vitamins
Choline chloride 140.0 0.75 0.005357143
D-Calcium pantothenate 477.0 0.75 0.001572327
Folic Acid 441.0 0.75 0.0017006802
Nicotinamide 122.0 0.75 0.006147541
Pyridoxal hydrochloride 204.0 0.75 0.0036764706
Riboflavin 376.0 0.075 1.994681E-4
Thiamine hydrochloride 337.0 0.75 0.0022255194
i-Inositol 180.0 1.5 0.008333334
Inorganic Salts
Magnesium Sulfate (MgSO4-7H2O) 246.0 150.0 0.6097561
Potassium Chloride (KCl) 75.0 300.0 4.0
Sodium Bicarbonate (NaHCO3) 84.0 1650.0 19.642857
Sodium Chloride (NaCl) 58.0 5100.0 87.93104
Sodium Phosphate dibasic (Na2HPO4-H2O) 138.0 1050.0 7.6086955
Other Components
D-Glucose (Dextrose) 180.0 750.0 4.1666665
FBS 250.0 Infinity
Heparin 50000.0 Infinity
Penicillin G, sodium 100000.0 Infinity
Phenol Red 398.0 7.5 0.01884422
Streptomycin Sulfate 100000.0 Infinity

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