Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)

Comparison of the photobleaching rates of the Alexa Fluor® 488 and Alexa Fluor® 546 dyes and the well-known fluorescein and Cy3 fluorophores. The cytoskeleton of bovine pulmonary artery endothelial cells (BPAEC) was labeled with (top series) Alexa Fluor® 488 phalloidin (Cat. no. A12379) and mouse monoclonal anti–alpha-tubulin antibody (Cat. no. A11126) in combination with Alexa Fluor® 546 goat anti–mouse IgG antibody (Cat. no. A11003) or (bottom series) fluorescein phalloidin (F432) and the anti–alpha-tubulin antibody in combination with a commercially available Cy3 goat anti–mouse IgG antibody. The pseudocolored images were taken at 30-second intervals (0, 30, 90, and 210 seconds of exposure from left to right). The images were acquired with bandpass filter sets appropriate for fluorescein and rhodamine.

Comparison of the photobleaching rates of the Alexa Fluor® 488 and Alexa Fluor® 546 dyes and the well-known fluorescein and Cy3 fluorophores. The cytoskeleton of bovine pulmonary artery endothelial cells (BPAEC) was labeled with (top series) Alexa Fluor® 488 phalloidin (Cat. no. A12379) and mouse monoclonal anti–alpha-tubulin antibody (Cat. no. A11126) in combination with Alexa Fluor® 546 goat anti–mouse IgG antibody (Cat. no. A11003) or (bottom series) fluorescein phalloidin (F432) and the anti–alpha-tubulin antibody in combination with a commercially available Cy3 goat anti–mouse IgG antibody. The pseudocolored images were taken at 30-second intervals (0, 30, 90, and 210 seconds of exposure from left to right). The images were acquired with bandpass filter sets appropriate for fluorescein and rhodamine.

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