Immunofluorescent Staining using Mouse anti-Golgin-97 Monoclonal Antibody.

HeLa cells. Anti–human Golgin-97, mouse monoclonal CDF4, Alexa Fluor® 430 goat anti–mouse IgG, Alexa Fluor® 594 phalloidin, and SYTOX® Green nucleic acid stain. Fixed, permeabilized and RNase-treated HeLa cells were labeled with mouse monoclonal anti–human Golgin-97 monoclonal antibody (Cat. no. A21270) and visualized with the yellow-fluorescent Alexa Fluor® 430 goat anti–mouse IgG antibody (Cat. no. A11063). The F-actin was labeled with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. no. A12381) and the nuclei were stained with the SYTOX® Green nucleic acid stain (Cat. no. S7020). This multiple-exposure image was acquired using a Nikon Diaphot fluorescent microscope equipped with a Quantix CCD camera.

HeLa cells. Anti–human Golgin-97, mouse monoclonal CDF4, Alexa Fluor® 430 goat anti–mouse IgG, Alexa Fluor® 594 phalloidin, and SYTOX® Green nucleic acid stain. Fixed, permeabilized and RNase-treated HeLa cells were labeled with mouse monoclonal anti–human Golgin-97 monoclonal antibody (Cat. no. A21270) and visualized with the yellow-fluorescent Alexa Fluor® 430 goat anti–mouse IgG antibody (Cat. no. A11063). The F-actin was labeled with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. no. A12381) and the nuclei were stained with the SYTOX® Green nucleic acid stain (Cat. no. S7020). This multiple-exposure image was acquired using a Nikon Diaphot fluorescent microscope equipped with a Quantix CCD camera.

Related Products

Related Images

A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go ›

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Endothelial cells. Go ›

Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›

1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›

Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›