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Fixed, permeabilized bovine pulmonary artery endothelial cells were labeled with Texas Red®-X phalloidin (Cat. no. T7471), which stains F-actin, and counterstained with DAPI (Cat. no. D1306, D3571, D21490). The panels show the unprocessed image (upper panel), after deconvolution (middle panel) and after deconvolution and three-dimensional reconstruction (lower panel). The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/).
