Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants, i.e., Digitalis sp. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications. The digoxigenin markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes for visualization and detection.
C23H34O5; DIG; Digitalis