STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colonyforming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.
Please enter the information below and press OK to send your cart to Core Services for purchase.