DCL3 Antibodies

Target Information

DCL3 is a nuclear, protein-coding gene in Arabidopsis thaliana that produces a large multidomain RNase III family endoribonuclease of the Dicer-like class. The DCL3 protein is organized in the canonical Dicer architecture, with an N-terminal DExD/H-box helicase module followed by a helicase C region, a DUF283 segment implicated in macromolecular interactions, a PAZ domain that recognizes the termini of double-stranded RNA substrates, and a C-terminal catalytic core containing tandem RNase III domains plus double-stranded RNA-binding domain(s) required for precise duplex cleavage. Functionally, DCL3 is essential for endogenous RDR2-dependent small interfering RNA biogenesis and is specialized for generating predominantly 24-nucleotide small interfering RNAs from double-stranded RNA precursors produced in the RNA-directed DNA methylation pathway; these 24-nucleotide products guide transcriptional silencing and epigenetic regulation of repetitive and heterochromatic loci through downstream effector complexes, and DCL3 activity is also implicated in methylation-mediated defense against DNA viruses via the same silencing machinery.

Synonyms

AT3G43920; ATDCL3; dicer-like 3