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Agrisera
DCL3/Dicer-like protein 3 Polyclonal Antibody
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FIGURE: 1 / 1
DCL3/Dicer-like protein 3 Antibody (AS12-2103) in WB
50 µg of total protein Arabidopsis thaliana total cell extract which came from the ground, frozen powder and has been directly transferred to 2 x LaemmLi sample buffer was separated on 15% SDS-PAGE and blotted over night at 64 m A to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1:2,000(1)and 1:500(2... View More
Product Details
AS12-2103
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence.
Conjugate
Form
Lyophilized
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 350 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
DCL3 is a nuclear, protein-coding gene in Arabidopsis thaliana that produces a large multidomain RNase III family endoribonuclease of the Dicer-like class. The DCL3 protein is organized in the canonical Dicer architecture, with an N-terminal DExD/H-box helicase module followed by a helicase C region, a DUF283 segment implicated in macromolecular interactions, a PAZ domain that recognizes the termini of double-stranded RNA substrates, and a C-terminal catalytic core containing tandem RNase III domains plus double-stranded RNA-binding domain(s) required for precise duplex cleavage. Functionally, DCL3 is essential for endogenous RDR2-dependent small interfering RNA biogenesis and is specialized for generating predominantly 24-nucleotide small interfering RNAs from double-stranded RNA precursors produced in the RNA-directed DNA methylation pathway; these 24-nucleotide products guide transcriptional silencing and epigenetic regulation of repetitive and heterochromatic loci through downstream effector complexes, and DCL3 activity is also implicated in methylation-mediated defense against DNA viruses via the same silencing machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT3G43920; ATDCL3; dicer-like 3
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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