Search
Invitrogen
Lymphotoxin beta Receptor Monoclonal Antibody (eBio3C8 (3C8)), PE, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
Lymphotoxin beta Receptor Antibody (12-5671-82) in Flow
Staining of NIH/3T3 cell line with 0.125 µg of Rat IgG1 kappa Isotype Control PE (Product # 12-4301-82) (blue histogram) or 0.125 µg of Anti-Mouse Lymphotoxin beta Receptor PE (purple histogram). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-5671-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio3C8 (3C8)
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The antibody reacts with mouse lymphotoxin-beta receptor (LTBR). Northern blot analysis of tissues from adult mice showed that expression levels of LTBR mRNA were strong in lung, liver, and kidney, moderate in heart and testes, but weak in brain, thymus, spleen, and lymph nodes. The tumor necrosis factor receptor-related protein is the human receptor for the heterotrimer of lymphotoxin-alpha and lymphotoxin-beta. This LT-alpha/LT-beta heterotrimer (LT-alpha1beta2) is assumed to take part in immunologic reactions by cell-cell contact, but does not bind to either TNFR1 (CD120alpha) or TNFR2 (CD120beta). LTBR is expressed by day 7 embryos, so it has been speculated that the LT-alpha/LT-beta receptor system may also have some function in early embryogenesis.
Applications Reported: This eBio3C8 (3C8) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio3C8 (3C8) antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) family of receptors. It is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. The protein specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of the encoded protein can trigger apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: LT-beta receptor; lymphotoxin-beta receptor; TNF receptor-related protein; TNFR-III; unnamed protein product
Gene Aliases: Ltar; LTbetaR; TNF-R-III; Tnfbr; TNFCR; TNFR-RP; TNFR2-RP; TNFRrp; Tnfrsf3
UniProt ID: (Mouse) P50284
Entrez Gene ID: (Mouse) 17000
immune response
myeloid dendritic cell differentiation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of JNK cascade
hematopoietic or lymphoid organ development
lymph node development
cellular response to mechanical stimulus
positive regulation of extrinsic apoptotic signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support