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Invitrogen
Lymphotoxin beta Receptor Monoclonal Antibody (eBio3C8 (3C8)), Functional Grade, eBioscience™
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FIGURE: 1 / 7
Lymphotoxin beta Receptor Antibody (16-5671-82) in Flow
Staining of NIH/3T3 cell line with 0.25 µg of Rat IgG1 Isotype Control Functional Grade Purified (Product # 16-4301-81) (open histogram) or 0.25 µg of Anti-Mouse Lymphotoxin beta Receptor Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.
Product Details
16-5671-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio3C8 (3C8)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The antibody reacts with mouse lymphotoxin-beta receptor (LTBR). Northern blot analysis of tissues from adult mice showed that expression levels of LTBR mRNA were strong in lung, liver, and kidney, moderate in heart and testes, but weak in brain, thymus, spleen, and lymph nodes. The tumor necrosis factor receptor-related protein is the human receptor for the heterotrimer of lymphotoxinalpha and lymphotoxin beta. This LT alpha/LTbeta heterotrimer (LT alpha 1/beta 2) is assumed to take part in immunologic reactions by cell-cell contact, but does not bind to either TNFR1 (CD120 alpha) or TNFR2 (CD120 beta). LTBR is expressed by day 7 embryos, so it has been speculated that the LT alpha/LT beta receptor system may also have some function in early embryogenesis.
Applications Reported: This eBio3C8 (3C8) antibody has been reported for use in flow cytometric analysis and to have agonistic activity (via activation of NK kappa B).
Applications Tested: This eBio3C8 (3C8) antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) family of receptors. It is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. The protein specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of the encoded protein can trigger apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: LT beta-R; LT-beta receptor; Lymphotoxin-beta receptor; TNF receptor-related protein; TNFR-III; Tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3
Gene Aliases: AI256028; CD18; Ltar; LTbetaR; Ltbr; TNF-R-III; Tnfbr; TNFCR; TNFR-RP; TNFR2-RP; TNFRrp; Tnfrsf3
UniProt ID: (Mouse) P50284
Entrez Gene ID: (Mouse) 17000
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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