Search

  • お問い合わせ一覧​
  • クイックオーダー

Invitrogen

ID2 Monoclonal Antibody (ILCID2), PE, eBioscience™

Advanced Verification
3 Published Figures
2 References
View all (19) ID2 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
ID2 Monoclonal Antibody (ILCID2), PE, eBioscience™

Product Details

12-9475-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1.0 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ILCID2

Immunogen

Purified recombinant fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli
View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735055

Product Specific Information

Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol.

Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: bHLHb26; cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; helix-loop-helix protein ID2; Id-2; Inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; inhibitor of DNA binding 2, HLH protein; MGC26389; OTTHUMP00000140258; OTTHUMP00000200297; unnamed protein product

View more View less

Gene Aliases: AI255428; BHLHB26; C78922; GIG8; Id-2; ID2; ID2A; ID2H; Idb2

View more View less

UniProt ID: (Human) Q02363, (Mouse) P41136

View more View less

Entrez Gene ID: (Human) 3398, (Mouse) 15902

View more View less

Function(s)
transcription corepressor activity protein binding ion channel binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding transcription regulator inhibitor activity DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter metanephros development natural killer cell differentiation thigmotaxis leukocyte differentiation membranous septum morphogenesis bundle of His development regulation of transcription from RNA polymerase II promoter heart development circadian rhythm adult locomotory behavior entrainment of circadian clock regulation of gene expression positive regulation of gene expression negative regulation of gene expression oligodendrocyte development regulation of lipid metabolic process olfactory bulb development neuron differentiation B cell differentiation erythrocyte differentiation circadian regulation of gene expression mammary gland epithelial cell proliferation regulation of circadian rhythm entrainment of circadian clock by photoperiod enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity locomotor rhythm negative regulation of B cell differentiation positive regulation of fat cell differentiation positive regulation of erythrocyte differentiation positive regulation of macrophage differentiation regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of blood pressure negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated cell development cell maturation rhythmic process Peyer's patch development embryonic digestive tract morphogenesis positive regulation of smooth muscle cell proliferation neuron fate commitment cell morphogenesis involved in neuron differentiation astrocyte differentiation oligodendrocyte differentiation positive regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation white fat cell differentiation negative regulation of muscle cell differentiation adipose tissue development mammary gland alveolus development epithelial cell differentiation involved in mammary gland alveolus development endodermal digestive tract morphogenesis cellular response to lithium ion dopaminergic neuron differentiation cellular senescence negative regulation of dopaminergic neuron differentiation regulation of G1/S transition of mitotic cell cycle regulation of neural precursor cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development positive regulation of cell proliferation negative regulation of DNA binding negative regulation of neuron differentiation positive regulation of cell cycle lymph node development positive regulation of cell cycle arrest negative regulation of neural precursor cell proliferation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support