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ID2 Monoclonal Antibody (ILCID2), eFluor™ 450, eBioscience™
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FIGURE: 1 / 6
ID2 Antibody (48-9475-82) in Flow
C57BL/6 mouse splenocytes stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with either 1.0 µg of Mouse IgG1 kappa Isotype Control, eFluor 450 (Product # 48-4714-82) (left) or 1.0 µg of ID2 Monoclonal Antibody, eFluor 450 (right). Cells were co-stained and gated based on the expression of both NK1.1 Monoclonal Antibody, APC (Product # 17-5941-82) and CD49b Monoclonal Antibody, APC (Product # 17-5971-82) (purple histogram); CD4 Monoclonal Antibody, FITC (Product # 11-0042-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
48-9475-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ILCID2
Immunogen
Purified recombinant fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli
Conjugate
eFluor™ 450
eFluor™ 450
eFluor™ 450
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol.
Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 450 is an alternative for Pacific Blue™. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Target Information
The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: cell growth-inhibiting gene 8; class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID2; helix-loop-helix protein ID2; Id-2; inhibitor of differentiation 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; inhibitor of DNA binding 2, HLH protein; MGC26389; OTTHUMP00000140258; OTTHUMP00000200297; unnamed protein product
Gene Aliases: bHLHb26; GIG8; ID2A; ID2H; Idb2
UniProt ID: (Human) Q02363, (Mouse) P41136
Entrez Gene ID: (Human) 3398, (Mouse) 15902
negative regulation of transcription from RNA polymerase II promoter
membranous septum morphogenesis
bundle of His development
regulation of transcription from RNA polymerase II promoter
heart development
circadian rhythm
positive regulation of gene expression
negative regulation of gene expression
regulation of lipid metabolic process
olfactory bulb development
neuron differentiation
circadian regulation of gene expression
mammary gland epithelial cell proliferation
regulation of circadian rhythm
entrainment of circadian clock by photoperiod
enucleate erythrocyte differentiation
locomotor rhythm
negative regulation of B cell differentiation
positive regulation of fat cell differentiation
positive regulation of macrophage differentiation
regulation of neuron differentiation
positive regulation of blood pressure
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
embryonic digestive tract morphogenesis
positive regulation of smooth muscle cell proliferation
neuron fate commitment
positive regulation of astrocyte differentiation
negative regulation of muscle cell differentiation
mammary gland alveolus development
epithelial cell differentiation involved in mammary gland alveolus development
endodermal digestive tract morphogenesis
cellular senescence
regulation of G1/S transition of mitotic cell cycle
regulation of neural precursor cell proliferation
metanephros development
natural killer cell differentiation
thigmotaxis
leukocyte differentiation
positive regulation of cell proliferation
adult locomotory behavior
entrainment of circadian clock
regulation of gene expression
oligodendrocyte development
cell differentiation
positive regulation of erythrocyte differentiation
negative regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of cell cycle
cell development
cell maturation
lymph node development
Peyer's patch development
cell morphogenesis involved in neuron differentiation
negative regulation of oligodendrocyte differentiation
adipose tissue development
cellular response to lithium ion
negative regulation of core promoter binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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