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Invitrogen
T-bet Monoclonal Antibody (eBio4B10 (4B10)), eBioscience™
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FIGURE: 1 / 53
T-bet Antibody (14-5825-82) in ICC/IF
Immunofluorescence analysis of T-bet was performed using 70% confluent log phase NK-92 cells. The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with T-bet Monoclonal Antibody (Product # 14-5825-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at a dilu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5825-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Published species
Bat,
Human,
Mouse,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBio4B10 (4B10)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
Applications Reported: This eBio4B10 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (with citrate antigen retrieval), immunoprecipitation, and immunoblotting (WB).
Applications Tested: This eBio4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 µg/mL as a starting dilution for western blotting.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor expressed in T cells; T-box transcription factor family member; Homo sapiens TBLYM homolog; T-cell-specific T-box transcription factor T-bet; transcription factor TBLYM; unnamed protein product
Gene Aliases: IMD88; T-bet; T-PET; TBET; TBLYM; TBT1
UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8
Entrez Gene ID: (Human) 30009, (Rhesus monkey) 694044, (Mouse) 57765
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell fate specification
T-helper 1 cell lineage commitment
leukocyte migration involved in inflammatory response
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to virus
negative regulation of interleukin-2 production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to dexamethasone
lymphocyte migration
negative regulation of T-helper 17 cell differentiation
negative regulation of T-helper 17 cell lineage commitment
negative regulation of T-helper 2 cell cytokine production
positive regulation of T-helper 1 cell cytokine production
positive regulation of gene expression
T cell differentiation
positive regulation of isotype switching to IgG isotypes
regulation of immune response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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