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T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor™ 660, eBioscience™
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FIGURE: 1 / 1
T-bet Antibody (606-5825-82) in Flow
Normal human peripheral blood cells were surface-stained with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0088-42). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.125 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 660 (Product # 606-4714-81) (left) or 0.125 µg of T-bet Monoclonal Antibody, Alexa Fluor 660 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 6... View More
Product Details
606-5825-82
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio4B10 (4B10)
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio4B10 (4B10) monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
Applications Reported: This eBio4B10 (4B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio4B10 (4B10) antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.
Target Information
T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor expressed in T cells; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM
Gene Aliases: IMD88; T-bet; T-PET; TBET; TBLYM; TBT1; TBX21
UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8
Entrez Gene ID: (Human) 30009, (Rhesus monkey) 694044, (Mouse) 57765
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription regulatory region DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell fate specification
T-helper 1 cell lineage commitment
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to virus
positive regulation of gene expression
T cell differentiation
negative regulation of interleukin-2 production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
regulation of immune response
lymphocyte migration
negative regulation of T-helper 17 cell differentiation
negative regulation of T-helper 17 cell lineage commitment
negative regulation of T-helper 2 cell cytokine production
positive regulation of T-helper 1 cell cytokine production
transcription, DNA-templated
cellular response to organic substance
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