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Invitrogen
Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), eBioscience™
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FIGURE: 1 / 3
Smooth Muscle Myosin Antibody (14-6400-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL Anti-Smooth Muscle Myosin Purified (right) followed by Anti-Mouse IgG HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-6400-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Cat,
Human,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SMMS-1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains.
Applications Reported: This SMMS-1 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This SMMS-1 antibody has been tested by western blot on rat heart and immunohistochemistry on formalin-fixed paraffin embedded tissue (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58). This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal perfomance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: contractile protein; epididymis secretory sperm binding protein; myosin heavy chain 21; myosin heavy chain SMA; myosin transcript variant (+) insert SM1; myosin transcript variant (+) insert SM2; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; myosin-11; SMMHC; smooth muscle myosin heavy chain
Gene Aliases: AAT4; FAA4; SMHC; SMMHC; SMMS-1; VSCM2
UniProt ID: (Human) P35749
Entrez Gene ID: (Bovine) 530050, (Human) 4629, (Dog) 479836, (Rat) 24582
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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