Search
Invitrogen
Actin (muscle) Monoclonal Antibody (HHF35), eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
Actin (muscle) Antibody (14-6496-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human pancreas using 10 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or 10 µg/mL Anti-Actin (muscle) Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-6496-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HHF35
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.
The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.
Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.
Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58) and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; unnamed protein product; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMDYS; SMGA; VSCM; VSCM1
UniProt ID: (Human) P68133, (Bovine) P68138, (Pig) P68137, (Bovine) P62739, (Human) P62736, (Bovine) Q3ZC07, (Human) P68032, (Human) P63261, (Bovine) P63258, (Bovine) Q5E9B5, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Horse) 100065454, (Human) 58, (Bovine) 281592, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Bovine) 515610, (Horse) 100062175, (Human) 59, (Dog) 478250, (Bovine) 533219, (Horse) 100057678, (Human) 70, (Pig) 100152267, (Dog) 475923, (Human) 71, (Bovine) 404122, (Pig) 397653, (Horse) 100050139, (Bovine) 281595, (Pig) 100520667, (Human) 72, (Horse) 100057561, (Dog) 475792, (Guinea pig) 100716933, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Guinea pig) 100722917, (Rat) 29275, (Guinea pig) 100731953, (Mouse) 11464, (Mouse) 11465, (Guinea pig) 100731713, (Rat) 287876, (Mouse) 11468, (Guinea pig) 100716255, (Rat) 25365
nucleotide binding
structural constituent of cytoskeleton
protein binding
ATP binding
hydrolase activity
myosin binding
ADP binding
protein kinase binding
microfilament motor activity
profilin binding
ubiquitin protein ligase binding
identical protein binding
structural constituent of postsynaptic actin cytoskeleton
ATPase activity
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
muscle contraction
positive regulation of gene expression
skeletal muscle thin filament assembly
skeletal muscle fiber development
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
positive regulation of ERK1 and ERK2 cascade
cellular response to transforming growth factor beta stimulus
juxtaglomerular apparatus development
positive regulation of hepatic stellate cell activation
actin filament organization
response to xenobiotic stimulus
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
cytoplasmic actin-based contraction involved in cell motility
angiogenesis
morphogenesis of a polarized epithelium
axonogenesis
positive regulation of cell migration
maintenance of blood-brain barrier
sarcomere organization
cell motility
regulation of stress fiber assembly
regulation of focal adhesion assembly
platelet aggregation
cellular response to interferon-gamma
positive regulation of wound healing
postsynaptic actin cytoskeleton organization
tight junction assembly
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
protein localization to bicellular tight junction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
metabolic process
response to drug
actin-mediated cell contraction
retina homeostasis
response to calcium ion
biological_process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support