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Invitrogen
Podoplanin Monoclonal Antibody (NZ-1.3), eBioscience™
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FIGURE: 1 / 16
Podoplanin Antibody (14-9381-82) in IHC (P)
Immunohistochemical analysis of Podoplanin was performed using formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-9381-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
NZ-1.3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis. This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma. Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex. Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2).
This antibody has been reported to block the interaction between Podoplanin and CLEC-2.
Applications Reported: This NZ-1.3 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This NZ-1.3 antibody has been tested by immunoblot analysis of U87 cells. This can be used at 1-5 µg per mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 43 kD glomerular glycoprotein; CTA-520D8.1; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; glycoprotein 36; glycoprotein 38; glycoprotein, 36-KD; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); lung type-I cell membrane-associated protein; membrane associated mucin-type glycoprotein; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RP23-348F1.2; T1-alpha; transmembrane mucin-type glycoprotein; type I cell 40 kDa protein; unnamed protein product
Gene Aliases: AGGRUS; D2-40; E11; GP36; Gp38; GP40; HT1A-1; OTS-8; OTS8; PA2.26; RTI140; RTI40; T1-alpha; T1A; T1A-2; T1A2; TI1A
UniProt ID: (Human) Q86YL7, (Rat) Q64294
Entrez Gene ID: (Human) 10630, (Rat) 54320
lymphangiogenesis
amino acid transport
cell adhesion
signal transduction
Rho protein signal transduction
negative regulation of cell proliferation
positive regulation of epithelial to mesenchymal transition
folic acid transport
cell migration
platelet activation
lung development
positive regulation of cell migration
negative regulation of apoptotic process
wound healing, spreading of cells
lymph node development
lymphatic endothelial cell fate commitment
actin-mediated cell contraction
positive regulation of extracellular matrix disassembly
regulation of substrate adhesion-dependent cell spreading
positive regulation of platelet aggregation
regulation of myofibroblast contraction
regulation of lamellipodium morphogenesis
prostaglandin metabolic process
nervous system development
cell proliferation
positive regulation of platelet activation
regulation of cell adhesion
tube morphogenesis
lung alveolus development
response to hyperoxia
cell-cell adhesion
regulation of G1/S transition of mitotic cell cycle
positive regulation of cell motility
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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