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Podoplanin Monoclonal Antibody (NZ-1.3), Alexa Fluor™ Plus 647
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FIGURE: 1 / 6
Podoplanin Antibody (756-9381-94) in IHC (P)
Immunohistochemical analysis of Podoplanin was performed on FFPE human colon adenocarcinoma tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 20-mins, followed by a 5-min cool down, a 1-min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30-mins, washed thric... View More
Product Details
756-9381-94
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
NZ-1.3
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis. This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma. Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex. Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2).
This antibody has been reported to block the interaction between Podoplanin and CLEC-2.
Applications Reported: This NZ-1.3 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This NZ-1.3 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aggrus; CTA-520D8.1; Glycoprotein 36; glycoprotein, 36-KD; Gp36; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); lung type-I cell membrane-associated protein; membrane associated mucin-type glycoprotein; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; T1-alpha; T1A; transmembrane mucin-type glycoprotein; unnamed protein product
Gene Aliases: AGGRUS; D2-40; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; PDPN; PSEC0003; PSEC0025; T1A; T1A-2; T1A2; TI1A
UniProt ID: (Human) Q86YL7
Entrez Gene ID: (Human) 10630
lymphangiogenesis
water transport
amino acid transport
cell adhesion
signal transduction
Rho protein signal transduction
negative regulation of cell proliferation
regulation of cell shape
positive regulation of epithelial to mesenchymal transition
folic acid transport
cell migration
platelet activation
lung development
positive regulation of cell migration
negative regulation of apoptotic process
wound healing, spreading of cells
lymph node development
lymphatic endothelial cell fate commitment
actin-mediated cell contraction
positive regulation of extracellular matrix disassembly
regulation of substrate adhesion-dependent cell spreading
positive regulation of platelet aggregation
regulation of myofibroblast contraction
regulation of lamellipodium morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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