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Invitrogen
Sall1 Monoclonal Antibody (NRNSTNX), eBioscience™
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FIGURE: 1 / 2
Sall1 Antibody (14-9729-82) in IHC (F)
Immunohistochemistry analysis of Sall1 in mouse brain tissue. Coronal cryosections of mouse brain tissue were fixed with ice cold acetone for 10 minutes and blocked with 10% mouse serum for 1hr at room temperature. The sections were stained with 1.25 µg /ml Sall1 Monoclonal Antibody overnight at 4C and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
14-9729-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
NRNSTNX
Immunogen
C-terminal fragment of the protein (aa-805-1322)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1. This clone has been reported to be successfully used in flow cytometry, native gel electrophoresis followed by western blot and immunohistochemistry of frozen tissue.
Applications Reported: This NRNSTNX antibody has been reported for use in native gel electrophoresis followed by western blot and in immunohistochemical staining.
Applications Tested: This NRNSTNX antibody has been tested by western blot (native) of mouse brain. This may be used at less than or equal to 5 µg/mL. This clone is not recommended for detection of denatured Sall1.
Applications Tested: This NRNSTNX antibody has been tested by immunohistochemical staining of acetone fixed frozen sections from mouse brain. This may be used at less than or equal to 1.25 µg/mL.
(Fluorochrome-conjugated NRNSTNX is recommended for use in flow cytometry)
Target Information
Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: sal-3; sal-like protein 1; zinc finger protein Spalt-3
Gene Aliases: Msal-3
Entrez Gene ID: (Mouse) 58198
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II transcription coactivator activity
enhancer sequence-specific DNA binding
nucleic acid binding
DNA binding
chromatin binding
histone deacetylase activity
protein binding
beta-catenin binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
neural tube closure
ventricular septum development
mesenchymal to epithelial transition involved in metanephros morphogenesis
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
heart development
olfactory nerve development
olfactory bulb development
olfactory bulb interneuron differentiation
neural tube development
positive regulation of Wnt signaling pathway
inductive cell-cell signaling
forelimb morphogenesis
hindlimb morphogenesis
outer ear morphogenesis
embryonic digit morphogenesis
positive regulation of neuron differentiation
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
limb development
olfactory bulb mitral cell layer development
kidney epithelium development
ureteric bud invasion
mesenchymal stem cell maintenance involved in metanephric nephron morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of neural precursor cell proliferation
negative regulation of mesoderm development
negative regulation of ectoderm development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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