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Invitrogen
CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), Functional Grade, eBioscience™
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FIGURE: 1 / 19
CD11b (activation epitope) Antibody (16-0113-82) in Flow
Cell death & disease 2023 -
Fig. 6 USP7 led to deubiquitination and stabilization of TRIM24. A Western blotting for detection of USP7, TRIM24, and SPLUNC1 levels in THP-1-derived Mphis after transfected with shUSP7 or shNC for 48 h. B M1 (CD86 + CD11b + ) macrophages in THP-1-derived Mphis transfected with shUSP7 or shNC was determined by flow cytometry. C Co-IP assay for confirming the protein interaction between USP7 and TRIM24 in T... View More
Product Details
16-0113-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CBRM1/5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.
Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis. CBRM1/5 has also been reported in blocking of CD11b function.
Applications Tested: The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; glycoprotein Mac-1; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion glycoprotein precursor; Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product
Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6
UniProt ID: (Human) Q4VAK1
Entrez Gene ID: (Human) 3684
microglial cell activation
immune system process
complement receptor mediated signaling pathway
response to ischemia
receptor-mediated endocytosis
phagocytosis, engulfment
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
response to mechanical stimulus
positive regulation of metabolic process
ectodermal cell differentiation
forebrain development
response to estradiol
positive regulation of superoxide anion generation
heterotypic cell-cell adhesion
positive regulation of neutrophil degranulation
innate immune response
negative regulation of dopamine metabolic process
positive regulation of protein targeting to membrane
beta-amyloid clearance
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
response to Gram-positive bacterium
complement-mediated synapse pruning
vertebrate eye-specific patterning
positive regulation of microglial cell mediated cytotoxicity
response to curcumin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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