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Invitrogen
alpha Tubulin Monoclonal Antibody (B-5-1-2)
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FIGURE: 1 / 58
alpha Tubulin Antibody (32-2500) in ICC/IF
Immunofluorescent analysis of Alpha - Tubulin was done on 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Alpha - Tubulin Mouse Monoclonal Antibody (Product # 32-2500) at 0.5 µg/mL in 1% BSA and incubated for 3 hours at... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
32-2500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat,
Sea urchin
Published species
Bovine,
Chicken,
Dog,
Hamster,
Human,
Mouse,
Plant,
Protozoa,
Rat,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
B-5-1-2
Immunogen
Sarkosyl-resistant filament from sea urchin sperm axonemes.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4 tubulin; alpha-1 tubulin; alpha-2; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); FLJ30169; H2 ALPHA; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUA; TUA1; TUA2; TUA3; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; tubulin alpha-3; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, beta 2
Gene Aliases: 2410129E14Rik; brdp; M[a]4; M[a]6; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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