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alpha Tubulin Monoclonal Antibody (B-5-1-2), Alexa Fluor™ 488
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FIGURE: 1 / 21
alpha Tubulin Antibody (322588) in ICC/IF
Immunofluorescence analysis of Alpha tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha tubulin (B-5-1-2) Alexa Fluor 488 Mouse Monoclonal Antibody, (Product # 322588) at 2µg/mL in 0.1% BSA a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
322588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Sea urchin
Published species
Chimpanzee,
Fungi,
Human,
Mouse,
Xenopus
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
B-5-1-2
Immunogen
Sarkosyl-resistant filament from sea urchin sperm axonemes
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with all forms of alpha tubulin. The expected molecular weight is ~50.5 kDa. Reactivity is confirmed with NIH3T3 cells, rat brain, mouse testis, human Caco-2, HeLa3 and PtK23 cells.
Excitation/Emission (nm) for this Alexa Fluor 488™-conjugated antibody: 495/519
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4 tubulin; alpha tubulin; alpha-1 tubulin; alpha-2; alpha-tubulin; Alpha-tubulin 1; alpha-tubulin 2; alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; alpha-tubulin 4; alpha-tubulin 6; alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; t beta-15; Talpha1 alpha-tubulin; TUA; TUA1; TUA2; TUA3; TUBA1/3/4; Tubulin alpha; tubulin alpha-1; Tubulin alpha-1 chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; tubulin alpha-3; tubulin alpha-3 chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; tubulin alpha-4 chain; tubulin alpha-6 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; tubulin H2-alpha; tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2; tubulin, beta 2B; tubulin, beta polypeptide paralog; unnamed protein product
Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; RGD1309427; RGD1565476; SPAX11; Tuba-1; TUBA1; TUBA2; TUBA3; Tuba4; TUBA6; Tubb2
UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) P68369, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Rat) Q5XIF6, (Mouse) P68368, (Mouse) Q3UX10, (Rat) Q3KRE8, (Mouse) Q9CWF2
Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Rat) 316531, (Mouse) 22145, (Rat) 291287, (Mouse) 238463, (Rat) 291081, (Mouse) 73710
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
microtubule binding
identical protein binding
macromolecular complex binding
protein heterodimerization activity
chaperone binding
double-stranded RNA binding
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
protein domain specific binding
microtubule cytoskeleton organization
mitotic cell cycle
cytoskeleton organization
microtubule-based process
neuron differentiation
cytoskeleton-dependent intracellular transport
cell division
neuron migration
positive regulation of axon guidance
startle response
protein folding
'de novo' protein folding
intracellular protein transport
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
sperm motility
adult behavior
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
protein stabilization
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
cellular response to interleukin-4
biological_process
modulation of synaptic transmission
embryonic brain development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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