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HELIOS Monoclonal Antibody (22F6), Brilliant Violet™ 480, eBioscience™
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FIGURE: 1 / 1
HELIOS Antibody (414-9883-42) in Flow
Swiss Webster mouse lymph node cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82), FOXP3 Monoclonal Antibody, APC (Product # 17-5773-82), and Armenian Hamster IgG Isotype Control, Brilliant Violet 480 (Product # 414-4888-81) (left) or HELIOS Monoclonal Antibody, Brilliant Violet 480 (right). Viable CD4-positive cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFl... View More
Product Details
414-9883-42
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
22F6
Conjugate
Excitation/Emission Max
437/478 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description
This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia. 22F6 has been reported to cross react with rat Helios.
Applications Tested
This 22F6 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse lymph node cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Ikaros family zinc finger protein 2; transcription factor; unnamed protein product; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios)
Gene Aliases: A730095J18Rik; ANF1A2; HELIOS; ICHAD; IKZF2; IMDIA; Zfpn1a2; ZNF1A2; ZNFN1A2
UniProt ID: (Human) Q9UKS7, (Mouse) P81183
Entrez Gene ID: (Dog) 488510, (Human) 22807, (Mouse) 22779
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
identical protein binding
metal ion binding
nucleic acid binding
protein homodimerization activity
protein heterodimerization activity
C2H2 zinc finger transcription factor
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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