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CD158a/h/g Monoclonal Antibody (HP-MA4), PerCP-Cyanine5.5, eBioscience™
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FIGURE: 1 / 1
CD158a/h/g Antibody (45-1589-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4732-82) (left) or Anti-Human CD158a/h/g PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
45-1589-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HP-MA4
Conjugate
Excitation/Emission Max
489/679 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody HP-MA4 recognizes several CD158 proteins, also known as KIR2D, specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g). It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d) or KIR2DS4 (CD158i) and crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) are unknown. CD158 proteins are members of the killer cell immunoglobulin-like receptors (KIR) family of transmembrane glycoproteins that are expressed on NK cells and a subset of T cells. Each KIR has an isoform with either a short (S) or long (L) cytoplasmic domain which transduces either an activating or inhibitory signal, respectively. The crossreactivity of the HP-MA4 antibody to KIR2DS3 and KIR2DS5 was published in 2013.
Applications Reported: This HP-MA4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HP-MA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD158; CD158 antigen-like family member A; CD158 antigen-like family member D; CD158 antigen-like family member G; CD158 antigen-like family member H; CD158 antigen-like family member I; CD158 antigen-like family member J; CD158a; CD158B1; CD158d; CD158g; CD158h; CD158i; CD158j; extracellular domain; G9P; Ig1-TM10A; Ig1-TM10A truncated; Ig2-TM9A Del; Killer cell immunoglobulin-like receptor 2DL1; Killer cell immunoglobulin-like receptor 2DL4; Killer cell immunoglobulin-like receptor 2DS1; Killer cell immunoglobulin-like receptor 2DS2; Killer cell immunoglobulin-like receptor 2DS4; Killer cell immunoglobulin-like receptor 2DS5; killer cell immunoglobulin-like receptor KIR2DS2; killer cell immunoglobulin-like receptor KIRDS1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; Killer cell inhibitory receptor 103AS; killer cell inhibitory receptor homolog; KIR homolog; killer Ig receptor; killer inhibitory receptor 2-2-1; killer inhibitory receptor 4-1-2; killer-cell Ig-like receptor; killer-cell immunoglobulin-like receptor; killer-cell immunoglobulin-like receptor two domains short tail 2 protein; KIR antigen 2DS4; KIR cl-17; killer cell inhibitory receptor; KIR-103AS; KIR2DL protein; KIR2DL1/3DL2; KIR2DL4; KIR2DL4 Killer-cell Immunoglobulin-like Receptor; KIR2DL4*00102; KIR2DL4*00103; KIR2DL4*00501; KIR2DL4*00801; KIR2DL4*00802; KIR2DL4*01101; KIR2DL4*021; KIR2DL4*022; KIR2DL4_00201; KIR2DL4_00201 protein; KIR2DS1 Killer-cell Immunoglobulin-like Receptor; KIR2DS2 Killer-cell Immunoglobulin-like Receptor; MHC class I NK cell receptor; MHC class I NK cell receptor Eb6 ActI; MHC class I NK cell receptor KIR103AS; natural killer associated transcript 5; natural killer cell inhibitory receptor; natural killer cell receptor; Natural killer-associated transcript 1; Natural killer-associated transcript 5; Natural killer-associated transcript 8; Natural killer-associated transcript 9; NK cell receptor; CD158d; NK receptor 183 ActI; NKAT-1; NKAT-5; NKAT-8; NKAT-9; NKAT5-delta-Ig1/Ig2; putative; nkat5-delta-Ig1; putative; NKAT6; nkat8; putative; nkat9; putative; p50 KAR; p50 killer cell activating receptor KAR-K1d; p50.3; p58 killer cell inhibitory receptor KIR-K64; p58 killer cell inhibitory receptor KIR-K7a; p58 KIR; p58 natural killer cell receptor clone CL-49; P58 natural killer cell receptor clones CL-39/CL-17; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6; p58 NK receptor CL-39/CL-17; p58 NK receptor CL-42/47.11; p58 NK receptor CL-49; p58.1 MHC class-I-specific NK receptor; short cytoplasmic tail variant; XXbac-BCX195L8.1
Gene Aliases: 183ActI; CD158A; CD158b; CD158D; CD158G; CD158H; CD158I; CD158J; cl-49; G9P; KIR-103AS; KIR-2DL4; KIR-2DS2; KIR-2DS4; KIR-K64; KIR103; KIR103AS; KIR1D; KIR221; KIR2DL1; KIR2DL3; KIR2DL4; KIR2DS1; KIR2DS2; KIR2DS4; KIR2DS5; KIR412; KKA3; NKAT; NKAT-1; NKAT-5; NKAT-8; NKAT1; NKAT5; NKAT8; NKAT9; p50.1; p58.1
UniProt ID: (Human) P43626, (Human) Q99706, (Human) Q14954, (Human) P43631, (Human) P43632, (Human) Q14953
Entrez Gene ID: (Human) 3802, (Human) 3805, (Human) 3806, (Human) 100132285, (Human) 3809, (Human) 3810
stimulatory killer cell immunoglobulin-like receptor signaling pathway
immune response-regulating signaling pathway
immune response
natural killer cell inhibitory signaling pathway
positive regulation of natural killer cell cytokine production
cellular defense response
signal transduction
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of cellular senescence
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