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CD11b/c Monoclonal Antibody (OX42), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 7
CD11b/c Antibody (46-0110-82) in Flow
Staining of rat splenocytes with Anti-Rat Granulocyte Marker FITC (Product # 11-0570-82) and 0.06 µg of Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (left) or 0.06 µg of Anti-Rat CD11b/c PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
46-0110-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX42
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4). CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.
The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages. Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation.
Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD11bc; CR3A; Integrin alpha M; integrin alpha-M; integrin alpha-X; integrin, alpha X; MAC-1; MAC1A; MGC117044; MO1A; OX-42; OX42; SLEB6
Gene Aliases: Cd11b; RGD1561123
Entrez Gene ID: (Rat) 25021, (Rat) 499271
leukocyte migration involved in inflammatory response
cell adhesion
leukocyte cell-cell adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
microglia development
single organismal cell-cell adhesion
neutrophil chemotaxis
cellular extravasation
activated T cell proliferation
heterotypic cell-cell adhesion
defense response to virus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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