Hamburger Menu Button
Thermo Fisher Scientific Logo
Se connecter
Vous n’avez pas de compte? Créer votre compte
  • Produits
    • Anticorps
    • Oligos, amorces, sondes et gènes
    • Réactions PCR en temps réel Taqman
    • Milieux de culture cellulaire
    • Produits chimiques
    • Colonnes et cartouches de chromatographie
    • Équipement de laboratoire
    • Consommables en plastique et fournitures de laboratoire
    • Microplaques
    • Produits plus écologiques
    • Afficher toutes les catégories de produits
  • Applications
    • Bioprocédés
    • Culture cellulaire et transfection
    • Thérapie cellulaire et génétique
    • Chromatographie
    • Tests moléculaires
    • Solutions numériques
    • Extraction et analyse d’ADN et d’ARN
    • Spectroscopie, analyse élémentaire et isotopique
    • Voir toutes les applications et techniques
  • Services
    • Services CDMO et CRO à 360°
    • Services CDMO
    • Services CRO
    • Services personnalisés
    • Services financiers et leasing
    • Services pour les instruments
    • Informatique de laboratoire
    • Offres commerciales et OEM
    • Services de formation
    • Unity Lab Services
    • Voir tous les services
  • Aide et assistance
    • S’inscrire et créer un compte
    • Comment commander
    • Assistance instruments
    • Centres d’assistance technique
    • Centres d’apprentissage
    • Consultez toutes les rubriques d'aide et d'assistance
  • Populaire
    • TaqMan Real-Time PCR Assays
      Reaction PCR en temps réel TaqMan
    • Antibodies
      Anticorps
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt - Synthèse Gène
    • Cell Culture Plastics
      Plastiques culture cellulaire
  • Nos clients
    • Biotechnologie
    • Industrie biopharmaceutique
    • CDMO
    • Diagnostics de laboratoire
    • Sciences industrielles et appliquées associées
  • Promotions
  • Contactez-nous
  • Commande rapide
  • Suivi et statut de la commande
  • Documents et certificats
Thermo Fisher Scientific Logo

Search

Rechercher
Search button
          • Statut des commandes
          • Commande rapide
          • Promos
          • Se connecter
            Se connecter
            Vous n’avez pas de compte? Créer votre compte
            • Mon compte
            • Commandes
            • Connect: lab, données, apps
            • Produits personnalisés et projets
            • Services Central
          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PerCP-eFluor™ 710, eBioscience™

          View all (80) SRC antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PerCP-eFluor™ 710, eBioscience™

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (1)
          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Phospho-SRC (Tyr418) Antibody (46-9034-42) in Flow

          Intracellular staining of mouse spleen cells untreated (orange histogram) or treated with staurosporine for 2 hours (purple histogram) with Anti-Human/Mouse phospho-Src (Y418) PerCP-eFluor® 710 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)

          Product Details

          46-9034-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.125 µg)/test
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Recommended Isotype Control

          Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SC1T2M3

          Conjugate

          PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710
          • Unconjugated
          • eFluor 660
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          482/708 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573866

          Product Specific Information

          Description: This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn.

          Specificity of this SC1T2M3 clone was determined by ELISA, flow cytometry, and western blotting.

          Applications Reported: This SC1T2M3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This SC1T2M3 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

          PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

          View more View less

          Gene Aliases: ASV; AW259666; c-SRC; p60-Src; pp60c-src; SRC; SRC1; THC6

          View more View less

          UniProt ID: (Human) P12931, (Mouse) P05480

          View more View less

          Entrez Gene ID: (Human) 6714, (Mouse) 20779

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding phospholipase activator activity kinase activity transferase activity enzyme binding protein domain specific binding heme binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding connexin binding scaffold protein binding protein kinase C binding insulin receptor binding protein C-terminus binding transferase activity, transferring phosphorus-containing groups protein kinase binding estrogen receptor binding protein complex binding cell adhesion molecule binding hormone receptor binding growth factor receptor binding
          Process(es)
          immune system process protein phosphorylation cell cycle cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway cell proliferation positive regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration phosphorylation cell migration peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation forebrain development positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of protein homooligomerization regulation of intracellular estrogen receptor signaling pathway adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis innate immune response bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation neurotrophin TRK receptor signaling pathway oogenesis positive regulation of cytokine secretion progesterone receptor signaling pathway positive regulation of protein transport regulation of cell cycle negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of intracellular signal transduction positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis negative regulation of transcription from RNA polymerase II promoter stimulatory C-type lectin receptor signaling pathway neutrophil activation involved in immune response negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly positive regulation of protein processing macroautophagy protein deubiquitination platelet activation T cell costimulation negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of telomere maintenance positive regulation of integrin activation toll-like receptor 3 signaling pathway regulation of toll-like receptor 3 signaling pathway positive regulation of dephosphorylation negative regulation of hippo signaling Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway angiotensin-activated signaling pathway TORC1 signaling vasodilation odontogenesis regulation of vascular permeability proteasome-mediated ubiquitin-dependent protein catabolic process regulation of bone resorption positive regulation of Notch signaling pathway positive regulation of glycolytic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway focal adhesion assembly leukocyte migration positive regulation of small GTPase mediated signal transduction intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of protein serine/threonine kinase activity regulation of heart rate by cardiac conduction positive regulation of protein localization to nucleus negative regulation of neutrophil activation negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of platelet-derived growth factor receptor-beta signaling pathway
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Commander Plus Icon Minus Icon
          • Statut des commandes
          • Assistance commandes
          • Commande rapide
          • Centre d’approvisionnement
          • Approvisionnement en ligne
          Assistance Plus Icon Minus Icon
          • Aide et assistance
          • Contactez-nous
          • Centres d’assistance technique
          • Documents et certificats
          • Signaler un problème sur le site
          Ressources Plus Icon Minus Icon
          • Centres d’apprentissage
          • Promotions
          • Événements et séminaires en ligne
          • Réseaux sociaux
          À propos de Thermo Fisher Plus Icon Minus Icon
          • À propos de nous À propos de nous
          • Emplois Emplois
          • Investisseurs Investisseurs
          • Actualités Actualités
          • Responsabilité sociale Responsabilité sociale
          • Marques commerciales
          • Politiques et notices d’information
          Notre portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          France flag icon
          France

          Your items have has been added!


          Host server : magellan-search-blue-67655d8755-b2k9d:80/100.66.79.173:80.
          git-commit: dddaa802cd395f65bf1581942d0e97a089c38f41
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.46.0-Offline