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Galectin 9 Monoclonal Antibody (RG9-35), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 2
Galectin 9 Antibody (46-9211-82) in Flow
Mouse bone marrow cells were surface stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.03 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321-82) (left) or 0.03 µg of Galectin 9 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9211-82) (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-9211-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RG9-35
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family. Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells.
Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Target Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gal9; Galectin9; HOM HD 21; lectin, galactose binding, soluble 5; unnamed protein product
Gene Aliases: gal-9; galectin-9; LGALS35; Lgals5
UniProt ID: (Mouse) O08573
Entrez Gene ID: (Mouse) 16859
positive regulation of cytokine production
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
transforming growth factor beta receptor signaling pathway
female pregnancy
positive regulation of gene expression
negative regulation of gene expression
regulation of T cell chemotaxis
response to lipopolysaccharide
negative regulation of interferon-gamma production
positive regulation of chemokine production
positive regulation of interleukin-1 production
positive regulation of interleukin-10 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
negative regulation of natural killer cell activation
regulation of natural killer cell differentiation
regulation of T cell differentiation in thymus
positive regulation of macrophage activation
receptor clustering
negative regulation of natural killer cell degranulation
positive regulation of innate immune response
maintenance of protein location
positive regulation of regulatory T cell differentiation
negative regulation of inflammatory response
positive regulation of oxidoreductase activity
positive regulation of SMAD protein import into nucleus
cellular response to virus
positive regulation of defense response to bacterium
regulation of T-helper 17 type immune response
positive regulation of T cell migration
negative regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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