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CXCL10 (IP-10) Monoclonal Antibody (4NY8UN), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 1
CXCL10 (IP-10) Antibody (46-9744-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Human IFN gamma Recombinant Protein (Product # 14-8319-80) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were intracellularly stained with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human CXCL10 (IP-10) PerCP-eFluor® 710 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte and monocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-9744-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
4NY8UN
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 4NY8UN monoclonal antibody reacts with human CXCL10. CXCL10, also known as Interferon Inducible Protein-10 (IP-10), is a member of the CXC family of chemokines. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. CXCL10 signaling is mediated by the G protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. In addition to its chemotactic functions, CXCL10 is a potent inhibitor of angiogenesis and tumor growth and modulates expression of adhesion molecules. Elevated levels of CXCL10 are observed in response to allergic inflammation, various infections, and Alzheimer's disease.
Applications Reported: This 4NY8UN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 4NY8UN antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; gamma IP10; H-IP-10; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10
Gene Aliases: C7; crg-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
UniProt ID: (Human) P02778
Entrez Gene ID: (Human) 3627
positive regulation of leukocyte chemotaxis
chemotaxis
defense response
inflammatory response
immune response
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
cell-cell signaling
muscle organ development
blood circulation
positive regulation of cell proliferation
response to gamma radiation
T cell chemotaxis
regulation of T cell chemotaxis
response to auditory stimulus
negative regulation of angiogenesis
positive regulation of cell migration
neutrophil chemotaxis
killing of cells of other organism
response to lipopolysaccharide
response to vitamin D
cellular response to heat
endothelial cell activation
regulation of cell proliferation
regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
positive chemotaxis
positive regulation of release of sequestered calcium ion into cytosol
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
cellular response to interleukin-17
cellular response to virus
antiviral innate immune response
regulation of endothelial tube morphogenesis
positive regulation of T cell migration
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