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CD68 Monoclonal Antibody (FA-11), APC-eFluor™ 780, eBioscience™
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FIGURE: 1 / 3
CD68 Antibody (47-0681-82) in Flow
Mouse bone marrow-derived macrophages were surface stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) followed by intracellular staining with 0.5 µg of Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or 0.5 µg of CD68 Monoclonal Antibody, APC-eFluor 780 (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
Product Details
47-0681-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FA-11
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin. CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP). Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells. CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. In the steady state, only about 10-15% of CD68 is present at the surface. Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling. CD68 binds oxidized LDL and is involved in the process of phagocytosis.
Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This FA-11 antibody has been tested by intracellular staining and flow cytometric analysis of mouse bone marrow derived macrophages using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD68; Macrosialin
Gene Aliases: Cd68; gp110; Lamp4; Scard1
UniProt ID: (Mouse) P31996
Entrez Gene ID: (Mouse) 12514
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