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Invitrogen

CD45RB Monoclonal Antibody (PD7/26), Alexa Fluor™ 700

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Product Details

56-9458-94

Applications
Tested Dilution
Publications

Multiplex Immunohistochemistry (mIHC)

20 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PD7/26

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Wet ice

RRID

AB_3679110

Product Specific Information

Description: The monoclonal antibody PD7/26 recognizes CD45RB, which is an isoform of the Leukocyte Common Antigen (LCA) also known as CD45. CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. Expression of CD45RB is more restricted than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been show to express low levels of CD45RB, which may correlate with increased migration to sites of infection. In contrast, high CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro.

Applications Reported: This PD7/26 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This PD7/26 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD45; CD45 antigen; glycoprotein 180; L-CA; Leukocyte common antigen; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; unnamed protein product

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Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)
phosphoprotein phosphatase activity protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity receptor binding protein binding heparin binding hydrolase activity protein kinase binding protein tyrosine kinase inhibitor activity ankyrin binding spectrin binding heparan sulfate proteoglycan binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade natural killer cell differentiation negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation positive regulation of immunoglobulin production positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion signal transduction cell surface receptor signaling pathway response to gamma radiation regulation of gene expression dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation regulation of interleukin-8 production negative regulation of interleukin-2 production positive regulation of interleukin-2 production positive regulation of tumor necrosis factor production heterotypic cell-cell adhesion T cell proliferation B cell proliferation positive regulation of T cell proliferation T cell activation gamma-delta T cell differentiation positive regulation of MAPK cascade cell cycle phase transition plasma membrane raft distribution positive thymic T cell selection negative thymic T cell selection positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of protein kinase activity negative regulation of JAK-STAT cascade alpha-beta T cell proliferation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development regulation of phagocytosis positive regulation of phagocytosis positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade extrinsic apoptotic signaling pathway negative regulation of interleukin-4-mediated signaling pathway negative regulation of microglial cell activation response to aldosterone DN2 thymocyte differentiation positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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