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Arginase 1 Antibody (56-9779-94) in IHC (P)
Immunohistochemical analysis of Arginase 1 was performed on formalin-fixed paraffin-embedded human liver (positive) and human tonsil (negative) tissues. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 1 (pH 6) for 15 min, followed by a 5 min cool down, a 1 min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized with 0... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
56-9779-94
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
sl6arg
Conjugate
Alexa Fluor™ 700
Alexa Fluor™ 700
Alexa Fluor™ 700
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.
Applications Reported: This sl6arg antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This sl6arg antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue and can be used at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-I; Arginase; arginase (EC 3.5.3.1); arginase 1 liver; arginase, liver; Arginase1; HGNC:663; Liver Arginase; liver-type arginase; Type 1 Arginase; type I arginase
UniProt ID: (Human) P05089
Entrez Gene ID: (Human) 383
urea cycle
arginine metabolic process
arginine catabolic process
regulation of cell proliferation
negative regulation of T cell proliferation
defense response to protozoan
negative regulation of activated T cell proliferation
negative regulation of interferon-gamma-mediated signaling pathway
neutrophil mediated killing of fungus
negative regulation of T-helper 2 cell cytokine production
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