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FIGURE: 1 / 2
DYKDDDDK Tag Antibody (701629RB) in WB
Western blot was performed using DYKDDDDK tag Recombinant Rabbit monoclonal antibody (8H8L17), Biotin (Product # 701629RB) by loading 30 µg modified whole cell extracts (1% SDS) of untransfected and transiently transfected HEK-293E lysates: Untransfected (Lane 1), empty vector control (Lane 2), HA-H3-DYKDDDDK (Lane 3), H3.3K4M-DYKDDDDK (Lane 4), H3.3K9M-DYKDDDDK (Lane 5), H3.3G34W-DYKDDDDK (Lane 6), DYKDDDDK-p65-HA (Lane 7), and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
701629RB
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H8L17
Immunogen
Synthetic peptide (DYKDDDDK)
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
DYKDDDDK Tag recombinant rabbit monoclonal antibody conjugated with Biotin specifically recognizes DYKDDDDK Tag at the C-terminus but not N-terminus of proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.
This antibody has been tested in western blot. The clone is reported to also work in immunoprecipitation (IP) assay, and it is recommended that the antibody be carefully titrated for optimal performance in this assay for best results.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Disclaimer
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