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DYKDDDDK Tag Antibody (701629RP647) in ICC/IF
Immunofluorescent analysis of DYKDDDDK Tag was performed using 70% confluent HA-H3-DYKDDDDK transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor™ Plus 647 (Product... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
701629RP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H8L17
Immunogen
Synthetic peptide (DYKDDDDK)
Conjugate
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
proprietary buffer, pH 6.5
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include better specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus antibodies. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Applications Tested: This DYKDDDDK Tag antibody has been tested by immunoblotting, immunocytochemistry and flow cytometric analysis of cells transfected with a vector encoding DYKDDDDK Tag. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
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