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FIGURE: 1 / 4
53BP1 Antibody (703580) in ICC/IF
Knockdown of 53BP1 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s14314 & s14315). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with 53BP1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-53BP1 Recombinant Rabbit Monoclonal Antibody (Product # 703580, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
703580
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H14L12
Immunogen
Protein corresponding to human 53BP1 [aa241-aa440]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1; unnamed protein product
Gene Aliases: 53BP1; p202; p53BP1; TDRD30; TP53BP1
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
p53 binding
DNA binding
damaged DNA binding
transcription cofactor activity
transcription coactivator activity
protein binding
telomeric DNA binding
histone binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitinated histone binding
histone H4K20me2 reader activity
histone reader activity
histone H4K20me methyltransferase activity
DNA damage checkpoint
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of isotype switching
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein homooligomerization
cellular response to X-ray
double-strand break repair via classical nonhomologous end joining
DNA strand resection involved in replication fork processing
negative regulation of DNA strand resection involved in replication fork processing
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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