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53BP1 Antibody (703580RP555) in ICC/IF
Immunofluorescent analysis of 53BP1 was performed using 70% confluent HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with 53BP1 Recombinant Rabbit Monoclonal Antibody (7H14L12) Alexa Fluor™ Plus 555 (Product # 703580RP555, 1:200) at 4 degree Celsius overnight. Panel a) shows representative images of cells that were stained for detection and localization of 53BP1. Panel b) shows repres... View More
Product Details
703580RP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H14L12
Immunogen
Protein corresponding to human 53BP1 [aa241-aa440]
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 53BP1 antibody has been tested by immunocytochemistry and flow cytometric analysis of HeLa cells. This may be used for immunocytochemistry at 5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
Gene Aliases: 53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
cellular response to DNA damage stimulus
protein sumoylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
cellular response to X-ray
Performance Guarantee
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