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FIGURE: 1 / 6
IL-17A Antibody (740021M) in ICC/IF
Immunofluorescent analysis of IL-17A was performed using 70% confluent Neuro-2A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IL-17A Recombinant Mouse Monoclonal Antibody (eBio64DEC17) (Product # 740021M, 1:100) at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32723) at a dilution of 1:2000 for 1 hour... View More
Product Details
740021M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
eBio64DEC17
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant Mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; inflammatory cytokine; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A; ILA17
UniProt ID: (Human) Q16552, (Mouse) Q62386
Entrez Gene ID: (Human) 3605, (Mouse) 16171
inflammatory response
positive regulation of necrotic cell death
positive regulation of interleukin-23 production
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
positive regulation of antimicrobial peptide production
adaptive immune response
immune system process
apoptotic process
immune response
Notch signaling pathway
cell-cell signaling
cell death
response to wounding
gene expression
positive regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-16 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
interleukin-17A-mediated signaling pathway
keratinocyte proliferation
innate immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive regulation of protein kinase B signaling
intestinal epithelial structure maintenance
regulation of primary metabolic process
interleukin-17-mediated signaling pathway
granulocyte migration
negative regulation of inflammatory response to wounding
positive regulation of bicellular tight junction assembly
positive regulation of tumor necrosis factor superfamily cytokine production
negative regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-X-C motif) ligand 1 production
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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