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Invitrogen
HuC/HuD Monoclonal Antibody (16A11), Biotin
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FIGURE: 1 / 34
HuC/HuD Antibody (A-21272) in IHC
Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Product # N-21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Product # S-11226). Both sections were counterstained with DAPI (Product # D1306, D3571, D21490) (Product # A-21272)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A-21272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fish,
Guinea pig,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
16A11
Immunogen
Human HuC/HuD neuronal protein.
Conjugate
Biotin
Biotin
Biotin
Form
Lyophilized
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.
Target Information
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV like neuron-specific RNA binding protein 3; ELAV like neuron-specific RNA binding protein 4; Hu antigen C; Hu antigen D; hu-antigen C; huc; paraneoplastic cerebellar degeneration-associated antigen; paraneoplastic encephalomyelitis antigen; paraneoplastic encephalomyelitis antigen HuD; paraneoplastic limbic encephalitis antigen 21; unnamed protein product
Gene Aliases: HUC; HUCL; HUD; PLE21; PNEM
UniProt ID: (Human) Q14576, (Human) P26378
Entrez Gene ID: (Human) 1995, (Human) 1996
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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