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CD106 (VCAM-1) Monoclonal Antibody (STA), eBioscience™

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Published Figures (12)

CD106 (VCAM-1) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

CD106 (VCAM-1) Antibody (14-1069-82) in ICC/IF

Nature communications 2017 - Figure 1 Enhanced expression of endothelial SENP1 and GATA2 correlates with graft arteriosclerosis (GA) progression. Similarly sized human coronary arteries with GA from chronically rejecting heart allografts or without disease from non-transplanted hearts were collected and evaluated by histological analysis. ( a , b ) Dramatically increased expression of endothelial SENP1 was detected in the diseased vess... View More

CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD106 (VCAM-1) Antibody in Western Blot, Immunoprecipitation (WB, IP)

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

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Product Details

14-1069-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 10 publications 10 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

STA

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467422

Product Specific Information

Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: STA has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNF alpha-activated HUVEC cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells.

PLoS genetics

Wünnemann F,Fotsing Tadjo T,Beaudoin M,Lalonde S,Lo KS,Kleinstiver BP,Lettre G

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.

Nature communications

Manriquez V,Nivoit P,Urbina T,Echenique-Rivera H,Melican K,Fernandez-Gerlinger MP,Flamant P,Schmitt T,Bruneval P,Obino D,Duménil G

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Differential Characterization of Temozolomide-Resistant Human Glioma Cells.

International journal of molecular sciences

Lai SW,Huang BR,Liu YS,Lin HY,Chen CC,Tsai CF,Lu DY,Lin C

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunofluorescence

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunofluorescence

Thu Jun 01 00:00:00 EDT 2017

TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways.

Molecular medicine reports

Lu ZY,Chen WC,Li YH,Li L,Zhang H,Pang Y,Xiao ZF,Xiao HW,Xiao Y

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

14-1069 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.

PloS one

Mahadev V,Starr R,Wright SL,Martinez C,Jensen MC,Barish ME,Forman SJ,Brown CE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunoprecipitation

Mon Dec 22 00:00:00 EST 2014

Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.

PloS one

Mahadev V,Starr R,Wright SL,Martinez C,Jensen MC,Barish ME,Forman SJ,Brown CE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunoprecipitation

Mon Dec 22 00:00:00 EST 2014

Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.

PloS one

Mahadev V,Starr R,Wright SL,Martinez C,Jensen MC,Barish ME,Forman SJ,Brown CE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunoprecipitation

Mon Dec 22 00:00:00 EST 2014

Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.

PloS one

Mahadev V,Starr R,Wright SL,Martinez C,Jensen MC,Barish ME,Forman SJ,Brown CE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunoprecipitation

Mon Dec 22 00:00:00 EST 2014

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2015

Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.

PloS one

Khanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S

14-1069 was used in Flow cytometry/Cell sorting to investigate hepatic differentiation capacity of menstrual stem cells compared to bone marrow-derived stem cells.

Sat Nov 08 00:00:00 EST 2014

Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells.

Microbial pathogenesis

Dietmann A,Millonig A,Combes V,Couraud PO,Kachlany SC,Grau GE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunofluorescence

Wed Feb 12 00:00:00 EST 2014

Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells.

Microbial pathogenesis

Dietmann A,Millonig A,Combes V,Couraud PO,Kachlany SC,Grau GE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1069-82) in Immunofluorescence

Wed Feb 12 00:00:00 EST 2014

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

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Gene Aliases: CD106; INCAM-100; VCAM1

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UniProt ID: (Human) P19320

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Entrez Gene ID: (Human) 7412

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Function(s)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Process(es)

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking B cell differentiation extracellular matrix organization response to lipopolysaccharide response to nicotine calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol regulation of immune response leukocyte tethering or rolling oxidation-reduction process cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to tumor necrosis factor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD106 (VCAM-1) Monoclonal Antibody (STA), eBioscience™

CD106 (VCAM-1) Antibody (14-1069-82) in ICC/IF

Product Details

14-1069-82

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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