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Invitrogen
CD11b Monoclonal Antibody (VIM12), FITC
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FIGURE: 1 / 4
CD11b Antibody (CD11B01) in Flow
Antigen Specificity: According to the literature this antibody recognizes the CD11b antigen. CD11b associates with CD18 to form the heterodimeric complex known as Mac-1. This complex serves as a receptor for the iC3b component. Mac-1 also serves as an adhesion molecule for intracellular adhesion molecule-1, also known as CD54. Mac-1 is expressed on cells of the myeloid lineage as well as natural killer cells.
Product Details
CD11B01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
VIM12
Immunogen
Human CD11b (Mac-1)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
Analyte Specific Reagent
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Bioinformatics
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; glycoprotein Mac-1; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion glycoprotein precursor; Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product
Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6
UniProt ID: (Human) Q4VAK1
Entrez Gene ID: (Human) 3684
microglial cell activation
immune system process
complement receptor mediated signaling pathway
response to ischemia
receptor-mediated endocytosis
phagocytosis, engulfment
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
response to mechanical stimulus
positive regulation of metabolic process
ectodermal cell differentiation
forebrain development
response to estradiol
positive regulation of superoxide anion generation
heterotypic cell-cell adhesion
positive regulation of neutrophil degranulation
innate immune response
negative regulation of dopamine metabolic process
positive regulation of protein targeting to membrane
beta-amyloid clearance
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
response to Gram-positive bacterium
complement-mediated synapse pruning
vertebrate eye-specific patterning
positive regulation of microglial cell mediated cytotoxicity
response to curcumin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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