Invitrogen

CD11b Monoclonal Antibody (M1/70), Super Bright™ 780, eBioscience™

Product Image Gallery

CD11b Antibody in Flow Cytometry (Flow) — BALB/c mouse bone marrow cells were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b kappa Isotype Control, Super Bright 780 (Product # 78-4031-82) (left) or 0.06 µg of CD11b Monoclonal Antibody, Super Bright 780 (right). Total viable cells were used for analysis.

CD11b Antibody in Immunocytochemistry (ICC/IF) — 10.1371/journal.pone.0255296.g003 Fig 3 Chronodisruption causes increased macrophage and microglial signaling in the placenta. Placentas from ( A-C ) control light (CL) and mice exposed to ( D-F ) developmental chronodisruption (CD) were labeled for inflammatory markers labeling microglia and macrophages within the decidual zone. In placenta from CD mice increased placental ( G ) Iba1 fluorescence (Student's 2-tailed unpaired t-test, t = 2.49, df = 8, p = 0.038) and increased placental (H ) CD11b fluorescence (Student's 2-tailed unpaired t-test, t = 2.70, df = 8, p = 0.027) were detected. CL = 4 placenta from different dams, 3 images each; CD = 6 placenta from different dams, 3 images each. All data are presented as mean +- SEM, scale bar = 20 microns, and * = p<0.05.

CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 5 Expression of IL-16 by CNS resident cells in EAE mice. Spinal cord and brain tissues were harvested from EAE and PBS control mice and stained with IL-16 (green) together with NeuN, GFAP or CD11b (all red). Representative images of spinal cord ( A - C ) and hippocampus ( D ) tissues are shown. Dotted white lines indicate tissue section edges. White arrows indicate areas of colocalisation. Percentage of double-positive cells in each group within either the spinal cord ( A - C ) or hippocampus ( E - G ) and cerebellum ( H - J ) was quantified with results expressed as the mean +- SEM. N = 5. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001 versus PBS or comparison groups are indicated by the bar lines.

CD11b Antibody in Immunohistochemistry (IHC) — Figure 4 Rb1 mitigates inflammation in Ang II-infused mice. C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 6.25 mg/kg (Rb1-L), 25 mg/kg (Rb1-M) and 100 mg/kg (Rb1-H). ( A ) Plasma was isolated and analyze by ELISA for the level of IL-1 beta, IL-6 and TNF (n=6-8 per group). ( B ) Immunohistochemistry (IHC) was performed to examine the presence of CD68 + cells (in red) in the heart. DAPI (in blue) was counterstained to help visualize the nuclei. Scale bar, 50 mum. ( C ) The number of CD68 + cells in the heart was quantified (n=5 per group). ( D ) IHC was performed to reveal the presence of CD11b + cells (in red) in the heart. DAPI (in blue) counterstaining was performed to visualize the nuclei. Scale bar, 50 mum. ( E ) The number of CD11b + cells in the heart was quantified (n=5 per group). ( F) . C57BL/6J mice were subcutaneously infused with Ang II (1 mug/kg/min) or saline (sham) for 14 days. Ang II-infused mice received daily treatment of either vehicle (Ang II) or Rb1 at 100 mg/kg (Rb1). The cardiac expression of Il1b, II6 , and Tnf was analyzed by real-time qPCR (n=6-7 per group). 18S rRNA was included as the internal control. Relative fold change was plotted against that from the sham controls. Data were expressed as mean+-S.E.M. * P<0.05, ** P<0.01, *** P<0.001.

CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Fig. 3 ZD55-IL-24 inhibits melanoma growth in B16-bearing immunocompetent mouse model mainly through the indirect pathway of inducing systemic antitumor immunity. A , B Changes of the immune-related cytokines in tumors treated with ZD55-IL-24. A Heat-map of the down-regulated immunosuppressive cytokines in Fig. 2 C . B Heat-map of the up-regulated immunostimulatory cytokines in Fig. 2 D . C - J Immunohistochemical analysis of immune cell infiltration in tumors, as well as recruitment and activation in spleens. Tumors and spleens resected from B16-bearing C57BL/6 mice receiving PBS or ZD55-IL-24 treatment indicated in Fig. 1 A were analyzed 2 days after the last injection by immunohistochemical staining. C Shown are representative images of tumor sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). D Quantification of the CD8 + cells in C ( n = 11). E Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in C ( n = 3). f Quantification of the NK1.1 + cells in c ( n = 6). G Quantification of the CD8 + cells in H ( n = 9). H Shown are representative images of spleen sections immunostained for CD8 (red, left panel), CD11b and Ly-6G (yellow, middle panel), and NK1.1 (red, right panel). I Quantification of the CD11b + Ly-6G + Ly-6C low neutrophils in H ( n = 3). j Quantification of NK1.1 + cells in h ( n = 9). Nuclei is counterstained with DAPI (blue). Data are mean +- SD. Scale bars, 300 um. Shown is one of t

CD11b Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Figure 3 Histochemical analysis of eye cross-sections show reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. (A) Histochemical analysis of eye cross-sections shows reduced rates of inflammatory cell infiltration and swelling of the cornea in Serp-1 treated mice. Enlarged images of the corneal region of two mice visibly show more swelling and a higher presence of inflammatory cells in the control mice. Inflammatory cell nuclei are stained by the deep-purple spots marked with white triangles in the light pink stromal layer of the cornea. (B) To further identify infiltrating immune cells, the slides were stained with CD11b (an immune cell marker, green). (C) CD11b positive cells (cells/0.1 mm 2 ) were quantified ( n = 3/group; * p < 0.05).

Product Details

78-0112-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Flow Cytometry (Flow)	0.125 µg/test	View 34 publications 34 publications
Neutralization (Neu)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	M1/70
Conjugate	Super Bright™ 780 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 eFluor 660 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Red 700 NovaFluor Yellow 570 NovaFluor Yellow 590 NovaFluor Yellow 660 PE PE-Cyanine5 PE-Cyanine7
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2722926

Product Specific Information

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dual Activity of Ginsenoside Rb1 in Hypertrophic Cardiomyocytes and Activated Macrophages: Implications for the Therapeutic Intervention of Cardiac Hypertrophy.

Journal of inflammation research

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Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Sat Apr 23 00:00:00 EDT 2022

Blockade of IDO-Kynurenine-AhR Axis Ameliorated Colitis-Associated Colon Cancer via Inhibiting Immune Tolerance.

Cellular and molecular gastroenterology and hepatology

Zhang X,Liu X,Zhou W,Du Q,Yang M,Ding Y,Hu R

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunocytochemistry

Fri Mar 25 00:00:00 EDT 2022

Developmental chronodisruption alters placental signaling in mice.

PloS one

Clarkson-Townsend DA,Bales KL,Hermetz KE,Burt AA,Pardue MT,Marsit CJ

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunocytochemistry

Wed Nov 24 00:00:00 EST 2021

Serp-1 Promotes Corneal Wound Healing by Facilitating Re-epithelialization and Inhibiting Fibrosis and Angiogenesis.

Frontiers in cardiovascular medicine

Ju B,Guo O,Benissan-Messan DZ,Shawver MH,Chen P,Geng B,Wei S,Yaron JR,Lucas AR,Zhu H

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry (PFA fixed)

Fri Jun 25 00:00:00 EDT 2021

Therapeutic Role of Inducible Nitric Oxide Synthase Expressing Myeloid-Derived Suppressor Cells in Acetaminophen-Induced Murine Liver Failure.

Frontiers in immunology

Hsu CY,Lin YC,Chang LY,Huang SK,Huang CH,Yang CK,Huang CT,Lin CY

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.

Biology

Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry (Frozen)

Thu May 27 00:00:00 EDT 2021

Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity.

Journal of immunology research

Antonucci L,Porcu C,Timperi E,Santini SJ,Iannucci G,Balsano C

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.

Nature communications

Jin H,Liu K,Tang J,Huang X,Wang H,Zhang Q,Zhu H,Li Y,Pu W,Zhao H,He L,Li Y,Zhang S,Zhang Z,Zhao Y,Qin Y,Pflanz S,Kasmi KEI,Zhang W,Liu Z,Ginhoux F,Ji Y,He B,Wang L,Zhou B

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunocytochemistry

Mon May 17 00:00:00 EDT 2021

Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance.

Frontiers in immunology

Hering L,Katkeviciute E,Schwarzfischer M,Busenhart P,Gottier C,Mrdjen D,Komuczki J,Wawrzyniak M,Lang S,Atrott K,Becher B,Rogler G,Scharl M,Spalinger MR

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Mon Apr 12 00:00:00 EDT 2021

Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages.

JCI insight

Hu X,Dong X,Li G,Chen Y,Chen J,He X,Sun H,Kim DH,Kemper JK,Chen LF

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Light Environment Influences Developmental Programming of the Metabolic and Visual Systems in Mice.

Investigative ophthalmology & visual science

Clarkson-Townsend DA,Bales KL,Marsit CJ,Pardue MT

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Lipin-1 Contributes to IL-4 Mediated Macrophage Polarization.

Frontiers in immunology

Chandran S,Schilke RM,Blackburn CMR,Yurochko A,Mirza R,Scott RS,Finck BN,Woolard MD

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Mon Mar 29 00:00:00 EDT 2021

Mycobacterium tuberculosis Load in Host Cells and the Antibacterial Activity of Alveolar Macrophages Are Linked and Differentially Regulated in Various Lung Lesions of Patients with Pulmonary Tuberculosis.

International journal of molecular sciences

Ufimtseva EG,Eremeeva NI,Umpeleva TV,Vakhrusheva DV,Skornyakov SN

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Fri Mar 26 00:00:00 EDT 2021

Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.

Cell host & microbe

Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed Feb 10 00:00:00 EST 2021

Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint.

Cells

Nazet U,Feulner L,Muschter D,Neubert P,Schatz V,Grässel S,Jantsch J,Proff P,Schröder A,Kirschneck C

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Mon Feb 01 00:00:00 EST 2021

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid-Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack.

BioMed research international

Jiang JK,Hong LJ,Lu YQ

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.

eLife

Kalinski AL,Yoon C,Huffman LD,Duncker PC,Kohen R,Passino R,Hafner H,Johnson C,Kawaguchi R,Carbajal KS,Jara JS,Hollis E,Geschwind DH,Segal BM,Giger RJ

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Wed Dec 02 00:00:00 EST 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

The armed oncolytic adenovirus ZD55-IL-24 eradicates melanoma by turning the tumor cells from the self-state into the nonself-state besides direct killing.

Cell death & disease

Hu HJ,Liang X,Li HL,Du CM,Hao JL,Wang HY,Gu JF,Ni AM,Sun LY,Xiao J,Hu JQ,Yuan H,Dai YS,Jin XT,Zhang KJ,Liu XY

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2020

The armed oncolytic adenovirus ZD55-IL-24 eradicates melanoma by turning the tumor cells from the self-state into the nonself-state besides direct killing.

Cell death & disease

Hu HJ,Liang X,Li HL,Du CM,Hao JL,Wang HY,Gu JF,Ni AM,Sun LY,Xiao J,Hu JQ,Yuan H,Dai YS,Jin XT,Zhang KJ,Liu XY

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2020

Loss of Atg7 causes chaotic nucleosome assembly of mouse bone marrow CD11b⁺Ly6G^- myeloid cells.

Aging

Fang Y,Gu Y,Li L,Zhu L,Qian J,Zhao C,Xu L,Wei W,Du Y,Yuan N,Zhang S,Yuan Y,Xu Y,Jiang C,Wang J

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Tue Nov 24 00:00:00 EST 2020

Exploring the Role of Osteosarcoma-Derived Extracellular Vesicles in Pre-Metastatic Niche Formation and Metastasis in the 143-B Xenograft Mouse Osteosarcoma Model.

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Mazumdar A,Urdinez J,Boro A,Arlt MJE,Egli FE,Niederöst B,Jaeger PK,Moschini G,Muff R,Fuchs B,Snedeker JG,Gvozdenovic A

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Fri Nov 20 00:00:00 EST 2020

Exploring the Role of Osteosarcoma-Derived Extracellular Vesicles in Pre-Metastatic Niche Formation and Metastasis in the 143-B Xenograft Mouse Osteosarcoma Model.

Cancers

Mazumdar A,Urdinez J,Boro A,Arlt MJE,Egli FE,Niederöst B,Jaeger PK,Moschini G,Muff R,Fuchs B,Snedeker JG,Gvozdenovic A

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Fri Nov 20 00:00:00 EST 2020

Divergent Effects of G2019S and R1441C LRRK2 Mutations on LRRK2 and Rab10 Phosphorylations in Mouse Tissues.

Cells

Iannotta L,Biosa A,Kluss JH,Tombesi G,Kaganovich A,Cogo S,Plotegher N,Civiero L,Lobbestael E,Baekelandt V,Cookson MR,Greggio E

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Thu Oct 22 00:00:00 EDT 2020

Electrotransfer of IL-15/IL-15Rα Complex for the Treatment of Established Melanoma.

Cancers

Shirley SA,Lundberg CG,Heller R

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Wed Oct 21 00:00:00 EDT 2020

Macrophage-Associated Lipin-1 Promotes β-Oxidation in Response to Proresolving Stimuli.

ImmunoHorizons

Schilke RM,Blackburn CMR,Rao S,Krzywanski DM,Finck BN,Woolard MD

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Mon Oct 19 00:00:00 EDT 2020

LRRK2 and Rab10 coordinate macropinocytosis to mediate immunological responses in phagocytes.

The EMBO journal

Liu Z,Xu E,Zhao HT,Cole T,West AB

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunocytochemistry

Thu Oct 15 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells.

Scientific reports

Rai V,Wood MB,Feng H,Schabla NM,Tu S,Zuo J

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

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Thu Aug 27 00:00:00 EDT 2020

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells.

Cancers

Kuziel G,Thompson V,D'Amato JV,Arendt LM

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Targeting miR-27a/VE-cadherin interactions rescues cerebral cavernous malformations in mice.

PLoS biology

Li J,Zhao Y,Choi J,Ting KK,Coleman P,Chen J,Cogger VC,Wan L,Shi Z,Moller T,Zheng X,Vadas MA,Gamble JR

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry (PFA fixed)

Mon Jun 01 00:00:00 EDT 2020

Irradiation induces cancer lung metastasis through activation of the cGAS-STING-CCL5 pathway in mesenchymal stromal cells.

Cell death & disease

Zheng Z,Jia S,Shao C,Shi Y

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Ly6c^Lo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation.

Scientific reports

Alkhani A,Levy CS,Tsui M,Rosenberg KA,Polovina K,Mattis AN,Mack M,Van Dyken S,Wang BM,Maher JJ,Nijagal A

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Tue Apr 28 00:00:00 EDT 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis.

Nature communications

Zhang X,Zhang H,Xu C,Li X,Li M,Wu X,Pu W,Zhou B,Wang H,Li D,Ding Q,Ying H,Wang H,Zhang H

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Fri Sep 13 00:00:00 EDT 2019

NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity.

PLoS biology

Gharagozloo M,Mahmoud S,Simard C,Yamamoto K,Bobbala D,Ilangumaran S,Smith MD,Lamontagne A,Jarjoura S,Denault JB,Blais V,Gendron L,Vilariño-Güell C,Sadovnick AD,Ting JP,Calabresi PA,Amrani A,Gris D

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis.

Autophagy

Qiang L,Sample A,Shea CR,Soltani K,Macleod KF,He YY

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Fri Jul 05 00:00:00 EDT 2019

Central nervous system regeneration is driven by microglia necroptosis and repopulation.

Nature neuroscience

Lloyd AF,Davies CL,Holloway RK,Labrak Y,Ireland G,Carradori D,Dillenburg A,Borger E,Soong D,Richardson JC,Kuhlmann T,Williams A,Pollard JW,des Rieux A,Priller J,Miron VE

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Distinct Tie2 tyrosine phosphorylation sites dictate phenotypic switching in endothelial progenitor cells.

Journal of cellular physiology

Siavashi V,Nassiri SM,Rahbarghazi R,Mohseni Z,Sharifi AM

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Chemokine Receptor Redundancy and Specificity Are Context Dependent.

Immunity

Dyer DP,Medina-Ruiz L,Bartolini R,Schuette F,Hughes CE,Pallas K,Vidler F,Macleod MKL,Kelly CJ,Lee KM,Hansell CAH,Graham GJ

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

The Outcome of the Cryptococcus neoformans-Macrophage Interaction Depends on Phagolysosomal Membrane Integrity.

Journal of immunology (Baltimore, Md. : 1950)

De Leon-Rodriguez CM,Rossi DCP,Fu MS,Dragotakes Q,Coelho C,Guerrero Ros I,Caballero B,Nolan SJ,Casadevall A

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Sun Jul 15 00:00:00 EDT 2018

Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury.

The Journal of clinical investigation

Lau A,Chung H,Komada T,Platnich JM,Sandall CF,Choudhury SR,Chun J,Naumenko V,Surewaard BG,Nelson MC,Ulke-Lemée A,Beck PL,Benediktsson H,Jevnikar AM,Snelgrove SL,Hickey MJ,Senger DL,James MT,Macdonald JA,Kubes P,Jenne CN,Muruve DA

78-0112 was used in Flow cytometry/Cell sorting to show that CI-AKI is a multistep process that involves immune surveillance by resident and infiltrating renal phagocytes, Nlrp3-dependent inflammation, and the tubular reabsorption of contrast via dipeptidase-1.

Mon Jul 02 00:00:00 EDT 2018

Morphine-potentiated cognitive deficits correlate to suppressed hippocampal iNOS RNA expression and an absent type 1 interferon response in LP-BM5 murine AIDS.

Journal of neuroimmunology

McLane VD,Kumar S,Leeming R,Rau S,Willis CL,Cao L

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunohistochemistry

Fri Jun 15 00:00:00 EDT 2018

MK2 contributes to tumor progression by promoting M2 macrophage polarization and tumor angiogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Suarez-Lopez L,Sriram G,Kong YW,Morandell S,Merrick KA,Hernandez Y,Haigis KM,Yaffe MB

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis.

Nature communications

Clemente C,Rius C,Alonso-Herranz L,Martín-Alonso M,Pollán Á,Camafeita E,Martínez F,Mota RA,Núñez V,Rodríguez C,Seiki M,Martínez-González J,Andrés V,Ricote M,Arroyo AG

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Neutralization

Fri Mar 02 00:00:00 EST 2018

Brd4 modulates the innate immune response through Mnk2-eIF4E pathway-dependent translational control of IκBα.

Proceedings of the National Academy of Sciences of the United States of America

Bao Y,Wu X,Chen J,Hu X,Zeng F,Cheng J,Jin H,Lin X,Chen LF

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Tue May 16 00:00:00 EDT 2017

Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.

Nature immunology

Fang J,Bolanos LC,Choi K,Liu X,Christie S,Akunuru S,Kumar R,Wang D,Chen X,Greis KD,Stoilov P,Filippi MD,Maciejewski JP,Garcia-Manero G,Weirauch MT,Salomonis N,Geiger H,Zheng Y,Starczynowski DT

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Immunocytochemistry

Wed Jun 01 00:00:00 EDT 2016

The cardioprotective protein apolipoprotein A1 promotes potent anti-tumorigenic effects.

The Journal of biological chemistry

Zamanian-Daryoush M,Lindner D,Tallant TC,Wang Z,Buffa J,Klipfell E,Parker Y,Hatala D,Parsons-Wingerter P,Rayman P,Yusufishaq MSS,Fisher EA,Smith JD,Finke J,DiDonato JA,Hazen SL

Published figure using CD11b monoclonal antibody (Product # 78-0112-82) in Flow Cytometry

Fri Jul 19 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

Entrez Gene ID: (Mouse) 16409

Molecular Function: integrin